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SYCP1

ANTIBODY INFORMATION
 
 

Antibody HPA021083

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA021083  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 5  

References

  

Proper citation

 Atlas Antibodies Cat#HPA021083, RRID:AB_1857685  

VALIDATION SUMMARY
 
 

Antibody HPA021083

  
Immunohistochemistry


Validated
  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA021083

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human testis shows nucleolar positivity in cells in seminiferous ducts.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 3 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:350  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA021083

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

 

Figure description

  

Target mass (kDa)

 114.2, 111.2, 93.1  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA021083

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA021083

  

Antigen

 Recombinant protein fragment  

Length (aa)

 125  

Antigen sequence

 
TLGGDSTFFKSFNKCTEDDFEFPFAKTNLSKNGENIDSDPALQKVNFLPV
LEQVGNSDCHYQEGLKDSDLENSEGLSRVYSKLYKEAEKIKKWKVSTEAE
LRQKESKLQENRKIIEAQRKAIQEL
 

Matching transcripts

 SYCP1-001 - ENSP00000358535 [100%]
SYCP1-002 - ENSP00000358531 [100%]
SYCP1-003 - ENSP00000410011 [100%]
SYCP1-201 - ENSP00000482532 [100%]
SYCP1-202 - ENSP00000480997 [100%]
 

Other gene match

  
ANTIGEN VIEW
SYCP1-001
SYCP1-002
SYCP1-003
SYCP1-201
SYCP1-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.