TOX - Antibodies - The Human Protein Atlas

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TOX

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA018322

Antibody HPA073241

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA018322

HPA073241

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA018322, RRID:AB_1858199

Atlas Antibodies Cat#HPA073241, RRID:AB_2686584

VALIDATION SUMMARY

Antibody HPA018322

Antibody HPA073241

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA018322

Antibody HPA073241

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.

Antibody dilution

1:46

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA018322

Antibody HPA073241

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Standard validation

Supported

Figure description

Immunohistochemical staining of human tonsil shows nuclear positivity in ggerminal center cells.

Expression

RNA: detected in 29 tissues
Protein: detected in 50 cell types

Retrieval

HIER pH6

Antibody dilution

1:250

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA018322

Antibody HPA073241

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

57.5

Antibody dilution

1:250

1:150

PROTEIN ARRAY

Antibody HPA018322

Antibody HPA073241

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:1850

ANTIGEN INFORMATION

Antibody HPA018322

Antibody HPA073241

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

PDAPCLGPSPCLDPYYCNKFDGENMYMSMTEPSQDYVPASQSYPGPSLES
EDFNIPPITPPSLPDHSLVHLNEVESGYHSLCHPMNHNGLLPFHPQN

PNNQMPVTVSIANMAVSPPPPLQISPPLHQHLNMQQHQPLTMQQPLGNQL
PMQVQ

Matching transcripts

TOX-001 - ENSP00000354842 [100%]

Other gene match

ANTIGEN VIEW

TOX-001

RELEVANT PUBLICATIONS

Antibody HPA018322

Antibody HPA073241

miR-223 regulates cell growth and targets proto-oncogenes in mycosis fungoides/cutaneous T-cell lymphoma
McGirt LY et al
J Invest Dermatol 2014;134(4):1101-7

TOX expression in cutaneous B-cell lymphomas
Schrader AM et al
Arch Dermatol Res 2016;308(6):423-7
Application: IHC

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.