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KIAA0753

ANTIBODY INFORMATION
 
 

Antibody HPA023057

  

Antibody HPA023494

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA023057   HPA023494  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   5  

References

 1   1  

Proper citation

 Atlas Antibodies Cat#HPA023057, RRID:AB_1852338   Atlas Antibodies Cat#HPA023494, RRID:AB_1852339  

VALIDATION SUMMARY
 
 

Antibody HPA023057

  

Antibody HPA023494

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA023057

  

Antibody HPA023494

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA023494.		  
Validated

Antibody staining overlaps with antibody HPA023057.		  
 

Standard validation

 Approved   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol & centrosome.   Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & centrosome.  

Antibody dilution

 1:50   1:200  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA023057

  

Antibody HPA023494

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA023494 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA023057 across 46 cell lines.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human gall bladder shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human gall bladder shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 77 cell types		   RNA: detected in 36 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:400  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA023057

  

Antibody HPA023494

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 109.4, 75.1   109.4, 75.1  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA023057

  

Antibody HPA023494

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA023057

  

Antibody HPA023494

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 112   104  

Antigen sequence

 
PCNGNSLDESVGTEEGSEKREAPLLSLAEDSQQKEGRAPLFVPPGMQHSI
GDYCSRFEQYLRIISHEAVGSFNPWLIAESFSEELVDEALGAVAAELQDM
CEDYAEAVFTSE
  
LKAEEMYRLQQLSVSATHLADKVEEAVLDRLKPLLVKAQRVNSTTEANIH
LKDGSSVNTAKAQPAQEVAAVDFESNNIRQLDDFLEDCASELWAVTHAKI
LGSE
 

Matching transcripts

 KIAA0753-001 - ENSP00000355250 [100%]
KIAA0753-002 - ENSP00000460050 [100%]
  KIAA0753-001 - ENSP00000355250 [100%]
KIAA0753-002 - ENSP00000460050 [100%]
 

Other gene match

    
ANTIGEN VIEW
KIAA0753-001
KIAA0753-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA023057

  

Antibody HPA023494

  

1.

 Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods
Jakobsen L et al
EMBO J 2011;30(8):1520-35
   Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods
Jakobsen L et al
EMBO J 2011;30(8):1520-35
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.