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Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
Antibody dilution
1:37
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA017986
Antibody HPA077490
Standard validation
Approved
Figure description
Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in seminiferus duct cells.
Expression
RNA: detected in 29 tissues Protein: detected in 57 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA017986
Antibody HPA077490
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
45.4
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA017986
Antibody HPA077490
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
