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ANTIBODY INFORMATION
 
 

Antibody HPA008736

  

Antibody HPA008797

  

Antibody HPA065779

  

Antibody CAB002224

  

Antibody CAB025546

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
   R&D Systems
  

Product name

 HPA008736   HPA008797   HPA065779   NCL-DAXX   AF4548  

Host species

 Rabbit   Rabbit   Rabbit   Mouse   Goat  

Clonality

 pAb   pAb   pAb   mAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Affinity  

Other gene match

          

Released in version

 3   3   14   1   5  

References

 9          

Proper citation

 Atlas Antibodies Cat#HPA008736, RRID:AB_1078625     Atlas Antibodies Cat#HPA065779, RRID:AB_2685558      

VALIDATION SUMMARY
 
 

Antibody HPA008736

  

Antibody HPA008797

  

Antibody HPA065779

  

Antibody CAB002224

  

Antibody CAB025546

  
Immunocytochemistry

 

Validated

Validated
  

Validated

Validated
      
Immunohistochemistry

Supported       Supported   Supported  
Western blot

Supported   Supported   Supported   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008736

  

Antibody HPA008797

  

Antibody HPA065779

  

Antibody CAB002224

  

Antibody CAB025546

  

Formal validation: Tagged protein

  
Validated

Antibody staining overlaps with GFP tagged protein		  
Validated

Antibody staining overlaps with GFP tagged protein		      

         

Figure description

   Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleus & nuclear bodies and GFP expression in nucleus & nuclear bodies.   Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleus & nuclear bodies and GFP expression in nucleus & nuclear bodies.      

Antibody dilution

   1:24   1:125      
 

Formal validation: Independent

  
Validated

Antibody staining overlaps with antibody HPA065779.		  
Validated

Antibody staining overlaps with antibody HPA008797.		      
 

Standard validation

   Supported   Supported      

         

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nuclear bodies.   Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleus & nuclear bodies.      

Antibody dilution

   1:20   1:125      

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008736

  

Antibody HPA008797

  

Antibody HPA065779

  

Antibody CAB002224

  

Antibody CAB025546

  

Standard validation

 Supported       Supported   Supported  

         

Figure description

 Immunohistochemical staining of human tonsil shows strong nuclear positivity in germinal center and non-germinal center cells.       Immunohistochemical staining of human lymph node shows strong cytoplasmic and nuclear positivity in reaction center cells and lymphoid cells outside reaction centra.   Immunohistochemical staining of human tonsil shows moderate nuclear positivity in reaction center cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 75 cell types		       RNA: detected in 35 tissues
Protein: detected in 63 cell types		   RNA: detected in 35 tissues
Protein: detected in 64 cell types		  

Retrieval

 HIER pH6       HIER pH6   HIER pH6  

Antibody dilution

 1:75       1:100   1:75  

Literature conformity

 Consistent with extensive gene/protein characterization data.       Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.       Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA008736

  

Antibody HPA008797

  

Antibody HPA065779

  

Antibody CAB002224

  

Antibody CAB025546

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

         

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY427987)		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 81.4, 76.3, 73.6   81.4, 76.3, 73.6   81.4, 76.3, 19.1   81.4, 76.3, 73.6, 19.1, 14.2, 4.8   81.4, 76.3, 73.6, 19.1, 14.2, 4.8  

Antibody dilution

 1:125   1:250   1:420   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA008736

  

Antibody HPA008797

  

Antibody HPA065779

  

Antibody CAB002224

  

Antibody CAB025546

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:500   1:500   1:5000      

ANTIGEN INFORMATION
 
 

Antibody HPA008736

  

Antibody HPA008797

  

Antibody HPA065779

  

Antibody CAB002224

  

Antibody CAB025546

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Not known   Recombinant protein  

Length (aa)

 146   146   136      

Antigen sequence

 
DEEEEAAAGKDGDKSPMSSLQISNEKNLEPGKQISRSSGEQQNKGRIVSP
SLLSEEPLAPSSIDAESNGEQPEELTLEEESPVSQLFELEIEALPLDTPS
SVETDISSSRKQSEEPFTTVLENGAGMVSSTSFNGGVSPHNWGDSG
  
DEEEEAAAGKDGDKSPMSSLQISNEKNLEPGKQISRSSGEQQNKGRIVSP
SLLSEEPLAPSSIDAESNGEQPEELTLEEESPVSQLFELEIEALPLDTPS
SVETDISSSRKQSEEPFTTVLENGAGMVSSTSFNGGVSPHNWGDSG
  
ARGSSSSGGKKCYKLENEKLFEEFLELCKMQTADHPEVVPFLYNRQQRAH
SLFLASAEFCNILSRVLSRARSRPAKLYVYINELCTVLKAHSAKKKLNLA
PAATTSNEPSGNNPPTHLSLDPTNAENTASQSPRTR
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					DAXX-001 - ENSP00000363668 [100%] 
DAXX-002 - ENSP00000266000 [100%] 
DAXX-201 - ENSP00000396876 [100%] 
DAXX-202 - ENSP00000482399 [100%] 

											 
																						DAXX-001 - ENSP00000363668 [100%] 
DAXX-002 - ENSP00000266000 [100%] 
DAXX-201 - ENSP00000396876 [100%] 
DAXX-202 - ENSP00000482399 [100%] 

											 
																						DAXX-001 - ENSP00000363668 [100%] 
DAXX-002 - ENSP00000266000 [100%] 
DAXX-202 - ENSP00000482399 [100%] 
DAXX-007 - ENSP00000406008 [97%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
DAXX-001
DAXX-002
DAXX-007
DAXX-201
DAXX-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA008736

												
										 
																				
											
											
											
Antibody HPA008797

												
										 
																				
											
											
											
Antibody HPA065779

												
										 
																				
											
											
											
Antibody CAB002224

												
										 
																				
											
											
											
Antibody CAB025546

												
										 
																		

																	

										
											
1.

												
																						Driver mutations in histone H3.3 and chromatin remodelling genes in paediatric glioblastoma

	Schwartzentruber J et al

	Nature 2012;482(7384):226-31

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Loss of ATRX or DAXX expression and concomitant acquisition of the alternative lengthening of telomeres phenotype are late events in a small subset of MEN-1 syndrome pancreatic neuroendocrine tumors

	de Wilde RF et al

	Mod Pathol 2012;25(7):1033-9

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																						Altered telomeres in tumors with ATRX and DAXX mutations

	Heaphy CM et al

	Science 2011;333(6041):425

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
4.

												
																						Sp100 provides intrinsic immunity against human papillomavirus infection

	Stepp WH et al

	MBio 2013;4(6):e00845-13

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
5.

												
																						Whole-exome sequencing identifies somatic ATRX mutations in pheochromocytomas and paragangliomas

	Fishbein L et al

	Nat Commun 2015;6:6140

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
6.

												
																						Epigenetic dysregulation and poorer prognosis in DAXX-deficient pancreatic neuroendocrine tumours

	Pipinikas CP et al

	Endocr Relat Cancer 2015;22(3):L13-8

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
7.

												
																						ATRX represses alternative lengthening of telomeres

	Napier CE et al

	Oncotarget 2015;6(18):16543-58

			Application: WB				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
8.

												
																						TERT promoter mutations are frequent and show association with MED12 mutations in phyllodes tumors of the breast

	Yoshida M et al

	Br J Cancer 2015;113(8):1244-8

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
9.

												
																						Exome Sequencing of Uterine Leiomyosarcomas Identifies Frequent Mutations in TP53, ATRX, and MED12

	Mäkinen N et al

	PLoS Genet 2016;12(2):e1005850

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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by the Knut & Alice Wallenberg foundation.