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RXRB

ANTIBODY INFORMATION
 
 

Antibody HPA063653

  

Antibody CAB002003

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Upstate
  

Product name

 HPA063653   06-527  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 14   1  

References

    

Proper citation

 Atlas Antibodies Cat#HPA063653, RRID:AB_2685076    

VALIDATION SUMMARY
 
 

Antibody HPA063653

  

Antibody CAB002003

  
Immunocytochemistry

Supported    
Immunohistochemistry

  Approved  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA063653

  

Antibody CAB002003

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleus.    

Antibody dilution

 1:31    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA063653

  

Antibody CAB002003

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.  

Expression

   RNA: detected in 34 tissues
Protein: detected in 76 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:100  

Literature conformity

   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA063653

  

Antibody CAB002003

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 57.4, 56.9   57.4, 56.9  

Antibody dilution

 1:100   1:500  

PROTEIN ARRAY
 
 

Antibody HPA063653

  

Antibody CAB002003

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1150    

ANTIGEN INFORMATION
 
 

Antibody HPA063653

  

Antibody CAB002003

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 31    

Antigen sequence

 
VAGGEQQTPEPEPGEAGRDGMGDSGRDSRSP
  

											 
																				

																		

										
											
Matching transcripts

												
																					RXRB-001 - ENSP00000363812 [100%] 
RXRB-002 - ENSP00000363817 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
RXRB-001
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