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Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:76
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA056878
Antibody HPA057019
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferus ducts.
Immunohistochemical staining of human testis shows no positivity.
Expression
RNA: detected in 33 tissues Protein: detected in 9 cell types
RNA: detected in 33 tissues Protein: detected in 5 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:300
1:250
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA056878
Antibody HPA057019
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
15.9
15.9
Antibody dilution
1:250
1:210
PROTEIN ARRAY
Antibody HPA056878
Antibody HPA057019
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3050
1:2550
ANTIGEN INFORMATION
Antibody HPA056878
Antibody HPA057019
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
51
54
Antigen sequence
FLQRHHPGAQAPDGRRKFGTDHVEVGSQAGADGTRPPKASLPPELQPPTN
C
