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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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HSPA1A
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    TISSUE

  • CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

HSPA1A

Gene description

Heat shock 70kDa protein 1A

Protein class

Cancer-related genes
Plasma proteins
Predicted intracellular proteins
Transporters

Predicted localization

Intracellular

Number of transcripts

2
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Cell line enriched (HEK93)
HPA (normal tissue):Mixed

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Ubiquitous cytoplasmic and nuclear expression.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data. Caution, targets protein from more than one gene.

Reliability score

Supported based on 3 antibodies.
HPA052504 , CAB017451 , CAB032815
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PROGNOSTIC SUMMARY

Prognostic marker in colorectal cancer (unfavourable).
Colorectal cancer p<0.001
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RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA052504
CAB017451
CAB032815

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Several cases of breast, ovarian and endometrial cancers showed moderate nuclear and cytoplasmic staining. Several cases of gliomas, melanomas, skin, liver, lung, stomach, urothelial and cervix cancers along with head and neck cancers displayed moderate nuclear positivity. Remaining cancer tissues were mainly negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most of the cancer tissues showed moderate cytoplasmic and nuclear positivity. Several breast and gastric cancers along with occasional urothelial and pancreatic cancers were strongly stained. Lymphomas and carcinoids were weakly stained or negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

A majority of malignant cells displayed moderate to strong cytoplasmic and nuclear positivity. Several non-Hodgkins lymphomas were weakly stained or negative.

GENE INFORMATION

Gene name

HSPA1A (HGNC Symbol)

Synonyms

HSP70-1, HSPA1

Description

Heat shock 70kDa protein 1A (HGNC Symbol)

Entrez gene summary

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]

Chromosome

6

Cytoband

p21.33

Chromosome location (bp)

31815464 - 31817946

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000204389 (version 83.38)

Entrez gene

3303

UniProt

P0DMV8 (UniProt - Evidence at protein level)

neXtProt

NX_P0DMV8

Antibodypedia

HSPA1A antibodies

PROTEIN BROWSER

HSPA1A-001
HSPA1A-002

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

HSPA1A-001
ENSP00000364802
ENST00000375651
P0DMV8 [Direct mapping]
Heat shock 70 kDa protein 1A
A8K5I0 [Target identity:100%; Query identity:100%]
Epididymis secretory protein Li 103; Heat shock 70kDa protein 1A; Heat shock 70kDa protein 1B; cDNA FLJ75127, highly similar to Homo sapiens heat shock 70kDa protein 1A, mRNA
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Transporters
   Transporter channels and pores
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
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GO:0000151 [ubiquitin ligase complex]
GO:0001618 [virus receptor activity]
GO:0001664 [G-protein coupled receptor binding]
GO:0005102 [receptor binding]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005814 [centriole]
GO:0005829 [cytosol]
GO:0005925 [focal adhesion]
GO:0010467 [gene expression]
GO:0010628 [positive regulation of gene expression]
GO:0016234 [inclusion body]
GO:0016887 [ATPase activity]
GO:0019899 [enzyme binding]
GO:0031072 [heat shock protein binding]
GO:0031397 [negative regulation of protein ubiquitination]
GO:0031625 [ubiquitin protein ligase binding]
GO:0032757 [positive regulation of interleukin-8 production]
GO:0034599 [cellular response to oxidative stress]
GO:0034605 [cellular response to heat]
GO:0042026 [protein refolding]
GO:0042623 [ATPase activity, coupled]
GO:0043488 [regulation of mRNA stability]
GO:0044183 [protein binding involved in protein folding]
GO:0046034 [ATP metabolic process]
GO:0046718 [viral entry into host cell]
GO:0048471 [perinuclear region of cytoplasm]
GO:0050821 [protein stabilization]
GO:0051082 [unfolded protein binding]
GO:0051092 [positive regulation of NF-kappaB transcription factor activity]
GO:0055131 [C3HC4-type RING finger domain binding]
GO:0060548 [negative regulation of cell death]
GO:0070370 [cellular heat acclimation]
GO:0070434 [positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway]
GO:0072562 [blood microparticle]
GO:0090084 [negative regulation of inclusion body assembly]
GO:1900034 [regulation of cellular response to heat]
GO:1901029 [negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway]
GO:1902236 [negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway]
GO:1902380 [positive regulation of endoribonuclease activity]
GO:1903265 [positive regulation of tumor necrosis factor-mediated signaling pathway]
GO:2001240 [negative regulation of extrinsic apoptotic signaling pathway in absence of ligand]
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641 aa
70.1 kDa
No 0
HSPA1A-002
ENSP00000477378
ENST00000608703
V9GZ37 [Direct mapping]
Heat shock 70 kDa protein 1A
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Predicted intracellular proteins
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476 aa
51.9 kDa
No 0

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