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PRRC2A

ANTIBODY INFORMATION
 
 

Antibody HPA046791

  

Antibody CAB026383

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA046791   3568.00.02  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 12   6  

References

    

Proper citation

 Atlas Antibodies Cat#HPA046791, RRID:AB_2679809    

VALIDATION SUMMARY
 
 

Antibody HPA046791

  

Antibody CAB026383

  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Approved    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA046791

  

Antibody CAB026383

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts.  

Expression

 RNA: detected in 23 tissues
Protein: detected in 59 cell types		   RNA: detected in 23 tissues
Protein: detected in 54 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:175   1:200  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA046791

  

Antibody CAB026383

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 228.9   228.9  

Antibody dilution

 1:260   1:500  

PROTEIN ARRAY
 
 

Antibody HPA046791

  

Antibody CAB026383

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2050    

ANTIGEN INFORMATION
 
 

Antibody HPA046791

  

Antibody CAB026383

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 90    

Antigen sequence

 
TDRGTEPGPIRPSHRPGPPVQFGTSDKDSDLRLVVGDSLKAEKELTASVT
EAIPVSRDWELLPSAAASAEPQSKNLDSGHCVPEPSSSGQ
   

Matching transcripts

 PRRC2A-001 - ENSP00000365175 [100%]
PRRC2A-002 - ENSP00000365201 [100%]
   

Other gene match

    
ANTIGEN VIEW
PRRC2A-001
PRRC2A-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.