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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cell junctions.
Antibody dilution
1:76
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA057194
Antibody CAB010162
Antibody CAB025656
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human small intestine shows cytoplasmic
positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 35 tissues Protein: detected in 77 cell types
RNA: detected in 35 tissues Protein: detected in 70 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:150
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA057194
Antibody CAB010162
Antibody CAB025656
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
