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MALRD1

ANTIBODY INFORMATION
 
 

Antibody HPA035732

  

Antibody HPA038106

  

Antibody HPA046848

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA035732   HPA038106   HPA046848  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 16   16   10  

References

      

Proper citation

 Atlas Antibodies Cat#HPA035732, RRID:AB_2674756   Atlas Antibodies Cat#HPA038106, RRID:AB_2675836   Atlas Antibodies Cat#HPA046848, RRID:AB_2679837  

VALIDATION SUMMARY
 
 

Antibody HPA035732

  

Antibody HPA038106

  

Antibody HPA046848

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

    Uncertain  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035732

  

Antibody HPA038106

  

Antibody HPA046848

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA038106.		  
Validated

Antibody staining overlaps with antibody HPA035732.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.   Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.    

Antibody dilution

 1:7   1:4    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035732

  

Antibody HPA038106

  

Antibody HPA046848

  

Standard validation

     Uncertain  

     

Figure description

     Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.  

Expression

     RNA: detected in 9 tissues
Protein: detected in 47 cell types		  

Retrieval

     HIER pH6  

Antibody dilution

     1:75  

Literature conformity

     No avaliable gene/protein characterization data.  

RNA consistency

     Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA035732

  

Antibody HPA038106

  

Antibody HPA046848

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 241, 165.4, 55.1   241, 165.4   241, 165.4  

Antibody dilution

 1:250   1:250   1:190  

PROTEIN ARRAY
 
 

Antibody HPA035732

  

Antibody HPA038106

  

Antibody HPA046848

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:500   1:1550  

ANTIGEN INFORMATION
 
 

Antibody HPA035732

  

Antibody HPA038106

  

Antibody HPA046848

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 63   87   87  

Antigen sequence

 
SSGSKEGSVARITTSKSFPASLGMCTVRFWFYMIDPRSMGILKVYTIEES
GLNILVWSVIGNK
  
DSTVIWRVLYNQGKQWLEATIQLGRLSQPFHLSLDKVSLGIYDGVSAIDD
IRFENCTLPLPAESCEGLDHFWCRHTRACIEKLRLCD
  
DSTVIWRVLYNQGKQWLEATIQLGRLSQPFHLSLDKVSLGIYDGVSAIDD
IRFENCTLPLPAESCEGLDHFWCRHTRACIEKLRLCD
 

Matching transcripts

 MALRD1-001 - ENSP00000366477 [100%]
MALRD1-002 - ENSP00000366476 [100%]
MALRD1-201 - ENSP00000412763 [100%]
  MALRD1-001 - ENSP00000366477 [100%]
MALRD1-201 - ENSP00000412763 [100%]
  MALRD1-001 - ENSP00000366477 [100%]
MALRD1-201 - ENSP00000412763 [100%]
 

Other gene match

      
ANTIGEN VIEW
MALRD1-001
MALRD1-002
MALRD1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.