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OLIG2

ANTIBODY INFORMATION
 
 

Antibody HPA003254

  

Antibody CAB019381

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA003254   2931.00.02  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 4   4  

References

    

Proper citation

 Atlas Antibodies Cat#HPA003254, RRID:AB_1854795    

VALIDATION SUMMARY
 
 

Antibody HPA003254

  

Antibody CAB019381

  
Immunocytochemistry

Approved    
Immunohistochemistry


Validated
   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003254

  

Antibody CAB019381

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.    

Antibody dilution

 1:74    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003254

  

Antibody CAB019381

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows weak nuclear positivity in glial cells.   Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in a subset of glial cells.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 4 cell types		   RNA: detected in 1 tissues
Protein: detected in 19 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1500   1:600  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003254

  

Antibody CAB019381

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 32.4   32.4, 6.1  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA003254

  

Antibody CAB019381

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA003254

  

Antibody CAB019381

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 113    

Antigen sequence

 
SPEPDDLFLPARSKGSSGSAFTGGTVSSSTPSDCPPELSAELRGAMGSAG
AHPGDKLGGSGFKSSSSSTSSSTSSAAASSTKKDKKQMTEPELQQLRLKI
NSRERKRMHDLNI
  

											 
																				

																		

										
											
Matching transcripts

												
																					OLIG2-001 - ENSP00000371794 [100%] 
OLIG2-002 - ENSP00000331040 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
OLIG2-001
OLIG2-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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