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Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA020136 across 71 cell types. Spearman correlation with HPA019132 across 68 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA020136 across 66 cell types. Spearman correlation with HPA019025 across 68 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA019132 across 66 cell types. Spearman correlation with HPA019025 across 71 cell types.
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human oral mucosa shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human oral mucosa shows strong positivity in squamous epithelial cells.
Immunohistochemical staining of human skin shows strong positivity in keratinocytes.
Expression
RNA: detected in 18 tissues Protein: detected in 39 cell types
RNA: detected in 18 tissues Protein: detected in 62 cell types
RNA: detected in 18 tissues Protein: detected in 53 cell types
RNA: detected in 18 tissues Protein: detected in 44 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:85
1:450
1:250
1:250
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019025
Antibody HPA019132
Antibody HPA020136
Antibody CAB009570
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
42.1, 25.8, 14.4
42.1
42.1, 25.8
42.1, 25.8, 14.4
Antibody dilution
1:250
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA019025
Antibody HPA019132
Antibody HPA020136
Antibody CAB009570
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
