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Immunohistochemical staining of human rectum shows distinct positivity in blood vessels.
Immunohistochemical staining of human bronchus shows capillary staining in stroma and membranous positivity in respiratory epithelial cells.
Expression
RNA: detected in 36 tissues Protein: detected in 10 cell types
RNA: detected in 36 tissues Protein: detected in 25 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:150
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA016421
Antibody HPA017672
Standard validation
Uncertain
No bands detected.
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400744)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400744)
Target mass (kDa)
35.7, 35.6
35.7, 35.6, 16.4
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA016421
Antibody HPA017672
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
