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PPP2R2A

ANTIBODY INFORMATION
 
 

Antibody HPA042122

  

Antibody HPA042770

  

Antibody HPA059497

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042122   HPA042770   HPA059497  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 PPP2R2C - ENSG00000074211 [95%]
PPP2R2B - ENSG00000156475 [100%]
PPP2R2D - ENSG00000175470 [100%]
   PPP2R2C - ENSG00000074211 [94%]
PPP2R2B - ENSG00000156475 [94%]
PPP2R2D - ENSG00000175470 [100%]
    

Released in version

 12   12   13  

References

      

Proper citation

 Atlas Antibodies Cat#HPA042122, RRID:AB_2677859   Atlas Antibodies Cat#HPA042770, RRID:AB_2678155   Atlas Antibodies Cat#HPA059497, RRID:AB_2684041  

VALIDATION SUMMARY
 
 

Antibody HPA042122

  

Antibody HPA042770

  

Antibody HPA059497

  
Immunocytochemistry

Approved      
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA042122

  

Antibody HPA042770

  

Antibody HPA059497

  

Standard validation

 Approved      

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol.      

Antibody dilution

 1:65      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042122

  

Antibody HPA042770

  

Antibody HPA059497

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows distinct cytoplasmic and nuclear positivity in neuronal cells.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 58 cell types		   RNA: detected in 37 tissues
Protein: detected in 33 cell types		   RNA: detected in 37 tissues
Protein: detected in 7 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:125   1:15  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Not consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042122

  

Antibody HPA042770

  

Antibody HPA059497

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 58.9, 58.2, 53, 52.1, 52, 51.7, 51.5, 51.4, 50.6, 50.4, 49.5   58.9, 58.2, 53, 52.1, 52, 51.7, 51.5, 51.4, 50.6, 50.4, 49.5, 23   53, 51.7, 3.4  

Antibody dilution

 1:250   1:250   1:80  

PROTEIN ARRAY
 
 

Antibody HPA042122

  

Antibody HPA042770

  

Antibody HPA059497

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:20000   1:600  

ANTIGEN INFORMATION
 
 

Antibody HPA042122

  

Antibody HPA042770

  

Antibody HPA059497

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 37   32   30  

Antigen sequence

 
YNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQQNA
  
FEEPEDPSSRSFFSEIISSISDVKFSHSGRYM
  
MEGAGGGNDIQWCFSQVKGAVDDDVAEADI
 

Matching transcripts

 PPP2R2A-001 - ENSP00000370113 [97%]
PPP2R2A-002 - ENSP00000325074 [97%]
  PPP2R2A-001 - ENSP00000370113 [97%]
PPP2R2A-002 - ENSP00000325074 [97%]
PPP2R2A-004 - ENSP00000430320 [97%]
  PPP2R2A-001 - ENSP00000370113 [97%]
PPP2R2A-002 - ENSP00000325074 [93%]
PPP2R2A-201 - ENSP00000486025 [87%]
 

Other gene match

 PPP2R2C - ENSG00000074211 [95%]
PPP2R2B - ENSG00000156475 [100%]
PPP2R2D - ENSG00000175470 [100%]
   PPP2R2C - ENSG00000074211 [94%]
PPP2R2B - ENSG00000156475 [94%]
PPP2R2D - ENSG00000175470 [100%]
    
ANTIGEN VIEW
PPP2R2A-001
PPP2R2A-002
PPP2R2A-004
PPP2R2A-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.