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Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in chief cells.
Expression
RNA: detected in 19 tissues Protein: detected in 9 cell types
RNA: detected in 19 tissues Protein: detected in 2 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:1500
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA046875
Antibody CAB026358
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
42, 27.9, 24.6, 24.5, 13.3
42, 27.9, 24.6, 19.7, 13.3
Antibody dilution
1:230
1:500
PROTEIN ARRAY
Antibody HPA046875
Antibody CAB026358
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
