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Immunofluorescent staining of human cell line HeLa shows localization to nuclear bodies.
Antibody dilution
1:130
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA001119
Antibody HPA030335
Standard validation
Approved
Figure description
Immunohistochemical staining of human appendix shows distinct nuclear positivity in lymphoid cells and glandular cells.
Expression
RNA: detected in 22 tissues Protein: detected in 61 cell types
Retrieval
HIER pH6
Antibody dilution
1:10
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001119
Antibody HPA030335
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401166)
Target mass (kDa)
44.7, 41.3
41.3
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA001119
Antibody HPA030335
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
