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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
Antibody dilution
1:13
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA001119
Antibody CAB009392
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human appendix shows distinct nuclear positivity in lymphoid cells and glandular cells.
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 61 cell types
RNA: detected in 37 tissues Protein: detected in 65 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:10
1:50
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001119
Antibody CAB009392
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401166)
Target mass (kDa)
44.7, 41.3
44.7, 20.7, 13, 3.7
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA001119
Antibody CAB009392
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
