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RP11-166B2.1

ANTIBODY INFORMATION
 
 

Antibody HPA053611

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA053611  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 RP11-231C14.4 - ENSG00000169203 [96%]
NPIPB3 - ENSG00000169246 [96%]
NPIPA1 - ENSG00000183426 [100%]
NPIPA5 - ENSG00000183793 [100%]
AC138969.4 - ENSG00000183889 [100%]
NPIPB4 - ENSG00000185864 [96%]
NPIPB15 - ENSG00000196436 [92%]
NPIPB9 - ENSG00000196993 [92%]
RP11-347C12.1 - ENSG00000198064 [96%]
NPIPB6 - ENSG00000198156 [92%]
NPIPA8 - ENSG00000214940 [100%]
NPIPA7 - ENSG00000214967 [100%]
NPIPA3 - ENSG00000224712 [100%]
RP11-1212A22.4 - ENSG00000233024 [100%]
NPIPB7 - ENSG00000233232 [92%]
NPIPB5 - ENSG00000243716 [96%]
NPIPB11 - ENSG00000254206 [92%]
NPIPA2 - ENSG00000254852 [100%]
NPIPB8 - ENSG00000255524 [92%]
  

Released in version

 11  

References

  

Proper citation

 Atlas Antibodies Cat#HPA053611, RRID:AB_2682206  

VALIDATION SUMMARY
 
 

Antibody HPA053611

  
Immunocytochemistry

Supported  
Immunohistochemistry

Supported  
Western blot

Supported  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA053611

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.  

Antibody dilution

 1:44  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA053611

  

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.  

Expression

 RNA: detected in 25 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:20  

Literature conformity

 No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA053611

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 129.2, 126.7, 126.5, 126, 125.2, 67.1, 65.9, 51.3, 49.6, 49.2, 48.1, 47.7, 47.2, 45.8, 45.6, 42.3, 42.2, 42.1, 40.6, 40.1, 40, 36.2, 35.3, 16.7  

Antibody dilution

 1:220  

PROTEIN ARRAY
 
 

Antibody HPA053611

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1750  

ANTIGEN INFORMATION
 
 

Antibody HPA053611

  

Antigen

 Recombinant protein fragment  

Length (aa)

 26  

Antigen sequence

 
NRMGHQPPPPTQQHSIIDNSLSLKTP
 

Matching transcripts

 RP11-166B2.1-001 - ENSP00000382101 [96%]
RP11-166B2.1-007 - ENSP00000448752 [96%]
 

Other gene match

 RP11-231C14.4 - ENSG00000169203 [96%]
NPIPB3 - ENSG00000169246 [96%]
NPIPA1 - ENSG00000183426 [100%]
NPIPA5 - ENSG00000183793 [100%]
AC138969.4 - ENSG00000183889 [100%]
NPIPB4 - ENSG00000185864 [96%]
NPIPB15 - ENSG00000196436 [92%]
NPIPB9 - ENSG00000196993 [92%]
RP11-347C12.1 - ENSG00000198064 [96%]
NPIPB6 - ENSG00000198156 [92%]
NPIPA8 - ENSG00000214940 [100%]
NPIPA7 - ENSG00000214967 [100%]
NPIPA3 - ENSG00000224712 [100%]
RP11-1212A22.4 - ENSG00000233024 [100%]
NPIPB7 - ENSG00000233232 [92%]
NPIPB5 - ENSG00000243716 [96%]
NPIPB11 - ENSG00000254206 [92%]
NPIPA2 - ENSG00000254852 [100%]
NPIPB8 - ENSG00000255524 [92%]
  
ANTIGEN VIEW
RP11-166B2.1-001
RP11-166B2.1-007
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.