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ACY1

ANTIBODY INFORMATION
 
 

Antibody HPA036174

  

Antibody HPA036175

  

Antibody CAB003695

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abnova
  

Product name

 HPA036174   HPA036175   H00000095-M01  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

 ABHD14A-ACY1 - ENSG00000114786 [100%]
   ABHD14A-ACY1 - ENSG00000114786 [100%]
    

Released in version

 10   10   2  

References

      

Proper citation

 Atlas Antibodies Cat#HPA036174, RRID:AB_2674988   Atlas Antibodies Cat#HPA036175, RRID:AB_2674989   Abnova Corporation Cat#H00000095-M01, RRID:AB_425286  

VALIDATION SUMMARY
 
 

Antibody HPA036174

  

Antibody HPA036175

  

Antibody CAB003695

  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Approved   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036174

  

Antibody HPA036175

  

Antibody CAB003695

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in renal tubules.   Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in subsets of renal tubules.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 6 cell types		   RNA: detected in 37 tissues
Protein: detected in 18 cell types		   RNA: detected in 37 tissues
Protein: detected in 2 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:2000   1:350   1:500  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036174

  

Antibody HPA036175

  

Antibody CAB003695

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 56.4, 45.9, 42.2, 38.6, 25.8   56.4, 45.9, 42.2, 37.6   45.9, 42.2, 38.6, 37.6, 25.8  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA036174

  

Antibody HPA036175

  

Antibody CAB003695

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA036174

  

Antibody HPA036175

  

Antibody CAB003695

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 79   86    

Antigen sequence

 
QGMELFVQRPEFHALRAGFALDEGIANPTDAFTVFYSERSPWWVRVTSTG
RPGHASRFMEDTAAEKLHKVVNSILAFRE
  
SVTSVNLTKLEGGVAYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWCQA
AGEGVTLEFAQKWMHPQVTPTDDSNPWWAAFSRVCK
  

											 
																				

																		

										
											
Matching transcripts

												
																					ACY1-001 - ENSP00000384296 [100%] 
ACY1-007 - ENSP00000417056 [100%] 
ACY1-006 - ENSP00000419262 [85%] 
ACY1-011 - ENSP00000419830 [85%] 

											 
																						ACY1-001 - ENSP00000384296 [100%] 
ACY1-007 - ENSP00000417056 [100%] 
ACY1-008 - ENSP00000417618 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					ABHD14A-ACY1 - ENSG00000114786 [100%]

											 
																						ABHD14A-ACY1 - ENSG00000114786 [100%]

											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ACY1-001
ACY1-006
ACY1-007
ACY1-008
ACY1-011
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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