PGAM5 - Antibodies - The Human Protein Atlas

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PGAM5

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA036978

Antibody HPA036979

Antibody CAB068215

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA036978

HPA036979

AMAb90803

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA036978, RRID:AB_10964347

Atlas Antibodies Cat#HPA036979, RRID:AB_10960559

Atlas Antibodies Cat#AMAb90803, RRID:AB_2665674

VALIDATION SUMMARY

Antibody HPA036978

Antibody HPA036979

Antibody CAB068215

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA036978

Antibody HPA036979

Antibody CAB068215

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.

Antibody dilution

1:200

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA036978

Antibody HPA036979

Antibody CAB068215

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA036979 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA036978 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA036979 across 72 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA036978 across 72 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Immunohistochemical staining of human tonsil shows strong granular cytoplasmic positivity in germinal center cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 69 cell types

RNA: detected in 37 tissues
Protein: detected in 64 cell types

RNA: detected in 37 tissues
Protein: detected in 69 cell types

Retrieval

HIER pH6

Antibody dilution

1:1200

1:100

1:350

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA036978

Antibody HPA036979

Antibody CAB068215

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

32, 28

32, 28, 15.9

Antibody dilution

1:250

1:1000

PROTEIN ARRAY

Antibody HPA036978

Antibody HPA036979

Antibody CAB068215

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:12000

1:500

ANTIGEN INFORMATION

Antibody HPA036978

Antibody HPA036979

Antibody CAB068215

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

NWDRREPLSLINVRKRNVESGEEELASKLDHYKAKATRHIFLIRHSQYHV
DGSLEKDRTLTPLGREQAELTGLRLASLGLKFNKIV

PGVCKVSTDLLREGAPIEPDPPVSHWKPEAVQYYEDGARIEAAFRNYIHR
ADARQEEDSYEIFICHANVIRYIVCSIPP

Matching transcripts

PGAM5-001 - ENSP00000321503 [100%]
PGAM5-005 - ENSP00000438465 [100%]

PGAM5-001 - ENSP00000321503 [100%]
PGAM5-004 - ENSP00000440372 [95%]
PGAM5-005 - ENSP00000438465 [95%]
PGAM5-006 - ENSP00000409537 [95%]

Other gene match

ANTIGEN VIEW

PGAM5-001	PGAM5-004	PGAM5-005	PGAM5-006

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.