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ACTN3

ANTIBODY INFORMATION
 
 

Antibody HPA006035

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA006035  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 ACTN1 - ENSG00000072110 [100%]
ACTN2 - ENSG00000077522 [83%]
ACTN4 - ENSG00000130402 [93%]
  

Released in version

 3  

References

  

Proper citation

 Atlas Antibodies Cat#HPA006035, RRID:AB_1078095  

VALIDATION SUMMARY
 
 

Antibody HPA006035

  
Immunohistochemistry

Uncertain  
Western blot

Supported  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006035

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.  

Expression

 RNA: detected in 3 tissues
Protein: detected in 74 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:350  

Literature conformity

 Not consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA006035

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

 

Figure description

 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 107.6, 107.1, 105.6, 104.9, 103.9, 103.2, 103.1, 102.7, 94.8, 29.6, 27.1  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA006035

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2000  

ANTIGEN INFORMATION
 
 

Antibody HPA006035

  

Antigen

 Recombinant protein fragment  

Length (aa)

 121  

Antigen sequence

 
SAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYG
KLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSS
FYHAFSGAQKAETAANRICKV
 

Matching transcripts

 ACTN3-001 - ENSP00000426797 [86%]
ACTN3-003 - ENSP00000422007 [86%]
 

Other gene match

 ACTN1 - ENSG00000072110 [100%]
ACTN2 - ENSG00000077522 [83%]
ACTN4 - ENSG00000130402 [93%]
  
ANTIGEN VIEW
ACTN3-001
ACTN3-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.