ACTN2 - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

ACTN2

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA006035

Antibody HPA008315

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA006035

HPA008315

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

ACTN1 - ENSG00000072110 [100%]
ACTN4 - ENSG00000130402 [93%]
ACTN3 - ENSG00000248746 [86%]

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA006035, RRID:AB_1078095

Atlas Antibodies Cat#HPA008315, RRID:AB_1844543

VALIDATION SUMMARY

Antibody HPA006035

Antibody HPA008315

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA006035

Antibody HPA008315

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.

Standard validation

Supported

Figure description

Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.

Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Expression

RNA: detected in 10 tissues
Protein: detected in 74 cell types

RNA: detected in 10 tissues
Protein: detected in 4 cell types

Retrieval

HIER pH6

Antibody dilution

1:350

1:1800

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA006035

Antibody HPA008315

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.

Figure description

Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

107.6, 107.1, 105.6, 104.9, 103.9, 103.2, 103.1, 102.7, 94.8, 29.6, 27.1

103.9, 79.7

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA006035

Antibody HPA008315

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2000

1:3000

ANTIGEN INFORMATION

Antibody HPA006035

Antibody HPA008315

Antigen

Recombinant protein fragment

Length (aa)

121

142

Antigen sequence

SAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYG
KLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSS
FYHAFSGAQKAETAANRICKV

AHEQFKATLPEADGERQSIMAIQNEVEKVIQSYNIRISSSNPYSTVTMDE
LRTKWDKVKQLVPIRDQSLQEELARQHANERLRRQFAAQANAIGPWIQNK
MEEIARSSIQITGALEDQMNQLKQYEHNIINYKNNIDKLEGD

Matching transcripts

ACTN2-201 - ENSP00000443495 [83%]
ACTN2-001 - ENSP00000355537 [82%]

ACTN2-001 - ENSP00000355537 [100%]
ACTN2-201 - ENSP00000443495 [100%]
ACTN2-202 - ENSP00000438384 [100%]

Other gene match

ACTN1 - ENSG00000072110 [100%]
ACTN4 - ENSG00000130402 [93%]
ACTN3 - ENSG00000248746 [86%]

ANTIGEN VIEW

ACTN2-001	ACTN2-201	ACTN2-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.