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Immunohistochemical staining of human tonsil shows distinct positivity in a subset of leukocytes.
Immunohistochemical staining of human tonsil shows distinct positivity in a subset of non-germinal cells.
Expression
RNA: detected in 5 tissues Protein: detected in 67 cell types
RNA: detected in 5 tissues Protein: detected in 48 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:175
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA004863
Antibody HPA052967
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406830)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
36.6, 27.4, 27.1, 25.7, 24.3, 24.2, 22.5
36.6, 27.2
Antibody dilution
1:250
1:1200
PROTEIN ARRAY
Antibody HPA004863
Antibody HPA052967
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
