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ASIC5

ANTIBODY INFORMATION
 
 

Antibody HPA014903

  

Antibody HPA029444

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA014903   HPA029444  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   6  

References

    

Proper citation

 Atlas Antibodies Cat#HPA014903, RRID:AB_1844498   Atlas Antibodies Cat#HPA029444, RRID:AB_10611671  

VALIDATION SUMMARY
 
 

Antibody HPA014903

  

Antibody HPA029444

  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA014903

  

Antibody HPA029444

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human colon shows membranous positivity in glandular cells.   Immunohistochemical staining of human prostate shows moderate cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 2 tissues
Protein: detected in 20 cell types		   RNA: detected in 2 tissues
Protein: detected in 46 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:200   1:30  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA014903

  

Antibody HPA029444

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 57.5   57.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA014903

  

Antibody HPA029444

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA014903

  

Antibody HPA029444

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 50   150  

Antigen sequence

 
AENGLLEKIKLCLSKKPLPSPTERKKFDHDFAISTSFHGIHNIVQNRSKI
  
EVQYVEKMEFPAVTFCNLNRFQTDAVAKFGVIFFLWHIVSKVLHLQEITA
NSTGSREATDFAASHQNFSIVEFIRNKGFYLNNSTLLDCEFFGKPCSPKD
FAHVFTEYGNCFTFNHGETLQAKRKVSVSGRGLSLLFNVNQEAFTDNPAL
 

Matching transcripts

 ASIC5-001 - ENSP00000442477 [100%]
  ASIC5-001 - ENSP00000442477 [100%]
 

Other gene match

    
ANTIGEN VIEW
ASIC5-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.