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C17orf49

ANTIBODY INFORMATION
 
 

Antibody HPA022961

  

Antibody HPA024457

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA022961   HPA024457  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 RNASEK-C17orf49 - ENSG00000161939 [93%]
   RNASEK-C17orf49 - ENSG00000161939 [100%]
  

Released in version

 5   5  

References

    

Proper citation

 Atlas Antibodies Cat#HPA022961, RRID:AB_1845592   Atlas Antibodies Cat#HPA024457, RRID:AB_1845593  

VALIDATION SUMMARY
 
 

Antibody HPA022961

  

Antibody HPA024457

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA022961

  

Antibody HPA024457

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.  

Antibody dilution

 1:137   1:100  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA022961

  

Antibody HPA024457

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 76 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:600  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA022961

  

Antibody HPA024457

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 24.8, 20, 17.9, 16.3   24.8, 20, 17.9, 17.4, 14  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA022961

  

Antibody HPA024457

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA022961

  

Antibody HPA024457

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 89   76  

Antigen sequence

 
TSASTKVGEIFSAAGAAFTKLGELTMQLHPVADSSPAGAKWTETEIEMLR
AAVKRFGDDLNHISCVIKERTVAQIKATVKRKVYEDSGI
  
ESPKKGPKKVASGVLSPPPAAPPPSSSSVPEAGGPPIKKQKADVTLSALN
DSDANSDVVDIEGLGETPPAKKLNFD
 

Matching transcripts

 C17orf49-001 - ENSP00000411851 [100%]
C17orf49-003 - ENSP00000447646 [100%]
C17orf49-006 - ENSP00000448598 [100%]
  C17orf49-001 - ENSP00000411851 [100%]
C17orf49-004 - ENSP00000448746 [100%]
C17orf49-005 - ENSP00000447063 [100%]
C17orf49-006 - ENSP00000448598 [100%]
 

Other gene match

 RNASEK-C17orf49 - ENSG00000161939 [93%]
   RNASEK-C17orf49 - ENSG00000161939 [100%]
  
ANTIGEN VIEW
C17orf49-001
C17orf49-003
C17orf49-004
C17orf49-005
C17orf49-006
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.