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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nuclear bodies & cytosol.
Antibody dilution
1:27
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA049940
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic, membranous and nuclear positivity in urothelial cells.
Expression
RNA: detected in 26 tissues Protein: detected in 72 cell types
Retrieval
HIER pH6
Antibody dilution
1:100
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA049940
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
24
Antibody dilution
1:130
PROTEIN ARRAY
Antibody HPA049940
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
