Fields »
ABOUT
HELP
BLOG
MRC1

ANTIBODY INFORMATION
 
 

Antibody HPA004114

  

Antibody HPA045134

  

Antibody CAB068203

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA004114   HPA045134   AMAb90746  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 4   12   13  

References

 4      

Proper citation

 Atlas Antibodies Cat#HPA004114, RRID:AB_1846270   Atlas Antibodies Cat#HPA045134, RRID:AB_2679228   Atlas Antibodies Cat#AMAb90746, RRID:AB_2665652  

VALIDATION SUMMARY
 
 

Antibody HPA004114

  

Antibody HPA045134

  

Antibody CAB068203

  
Immunohistochemistry


Validated
   Supported   Supported  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004114

  

Antibody HPA045134

  

Antibody CAB068203

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		      

     

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.      
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human lung shows cytoplasmic positivity in macrophages.   Immunohistochemical staining of human lung shows strong membranous and cytoplasmic positivity in macrophages.   Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in Kupffers cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 7 cell types		   RNA: detected in 35 tissues
Protein: detected in 10 cell types		   RNA: detected in 35 tissues
Protein: detected in 1 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:550   1:400   1:4000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA004114

  

Antibody HPA045134

  

Antibody CAB068203

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 166   166   166  

Antibody dilution

 1:250   1:250   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA004114

  

Antibody HPA045134

  

Antibody CAB068203

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA004114

  

Antibody HPA045134

  

Antibody CAB068203

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 115   108    

Antigen sequence

 
NEDHKRCVDAVSPSAVQTAACNQDAESQKFRWVSESQIMSVAFKLCLGVP
SKTDWVAITLYACDSKSEFQKWECKNDTLLGIKGEDLFFNYGNRQEKNIM
LYKGSGLWSRWKIYG
  
YCGELKGDPTMSWNDINCEHLNNWICQIQKGQTPKPEPTPAPQDNPPVTE
DGWVIYKDYQYYFSKEKETMDNARAFCKRNFGDLVSIQSESEKKFLWKYV
NRNDAQSA
  

											 
																				

																		

										
											
Matching transcripts

												
																					MRC1-001 - ENSP00000455897 [100%] 

											 
																						MRC1-001 - ENSP00000455897 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MRC1-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA004114

												
										 
																				
											
											
											
Antibody HPA045134

												
										 
																				
											
											
											
Antibody CAB068203

												
										 
																		

																	

										
											
1.

												
																						Mitochondrial targeted peptides attenuate residual myocardial damage after reversal of experimental renovascular hypertension

	Eirin A et al

	J Hypertens 2014;32(1):154-65

			Application: IHC				
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Renal vein cytokine release as an index of renal parenchymal inflammation in chronic experimental renal artery stenosis

	Eirin A et al

	Nephrol Dial Transplant 2014;29(2):274-82

			Application: IHC				
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																						In vivo characterization of alveolar and interstitial lung macrophages in rhesus macaques: implications for understanding lung disease in humans

	Cai Y et al

	J Immunol 2014;192(6):2821-9

			Application: ICC-IF				
											 
																						
											 
																						
											 
																				

																		

										
											
4.

												
																						Clinical and Pathological Findings Associated with Aerosol Exposure of Macaques to Ricin Toxin

	Pincus SH et al

	Toxins (Basel) 2015;7(6):2121-33

			Application: IHC				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.