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EPPK1

ANTIBODY INFORMATION
 
 

Antibody HPA066808

  

Antibody HPA067357

  

Antibody HPA069333

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA066808   HPA067357   HPA069333  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 16   16   14  

References

      

Proper citation

 Atlas Antibodies Cat#HPA066808, RRID:AB_2685726   Atlas Antibodies Cat#HPA067357, RRID:AB_2685826   Atlas Antibodies Cat#HPA069333, RRID:AB_2686123  

VALIDATION SUMMARY
 
 

Antibody HPA066808

  

Antibody HPA067357

  

Antibody HPA069333

  
Immunocytochemistry


Validated
  

Validated
  

Validated
  
Immunohistochemistry

    Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA066808

  

Antibody HPA067357

  

Antibody HPA069333

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA069333.
Antibody staining overlaps with antibody HPA067357.		  
Validated

Antibody staining overlaps with antibody HPA069333.
Antibody staining overlaps with antibody HPA066808.		  
Validated

Antibody staining overlaps with antibody HPA067357.
Antibody staining overlaps with antibody HPA066808.		  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to intermediate filaments.   Immunofluorescent staining of human cell line HeLa shows localization to intermediate filaments.   Immunofluorescent staining of human cell line MCF7 shows localization to intermediate filaments.  

Antibody dilution

 1:200   1:105   1:98  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA066808

  

Antibody HPA067357

  

Antibody HPA069333

  

Standard validation

     Approved  

     

Figure description

     Immunohistochemical staining of human skin shows moderate cytoplasmic positivity in epidermal cells.  

Expression

     RNA: detected in 10 tissues
Protein: detected in 27 cell types		  

Retrieval

     HIER pH6  

Antibody dilution

     1:2000  

Literature conformity

     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

     Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA066808

  

Antibody HPA067357

  

Antibody HPA069333

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

     

Figure description

      

Target mass (kDa)

 555.7, 553.1   555.7, 553.1   555.7, 553.1  

Antibody dilution

 1:1200   1:350   1:330  

PROTEIN ARRAY
 
 

Antibody HPA066808

  

Antibody HPA067357

  

Antibody HPA069333

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:14400   1:4200   1:3900  

ANTIGEN INFORMATION
 
 

Antibody HPA066808

  

Antibody HPA067357

  

Antibody HPA069333

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 74   90   88  

Antigen sequence

 
LWELLFSEAISSEQRAMLAQQYQEGTLSVEKLAAKLSATLEQAAATARVT
FSGLRDTVTPGELLKAEIIDQDLY
  
STGRFKGCSVSLWDLLLSEYVGADKRRELVALCRSGRAAALRQVVSAVTT
LVEAAERQPLQATFRGLRKQVSARDLFRAQLISRKTLDEL
  
ECPRDETSGLHLLPLPESAPALPTEEQVQRSLQAVPGAKDGTSLWDLLSS
CHFTEEQRRGLLEDVQEGRTTVPQLLASVQRWVQETKL
 

Matching transcripts

 EPPK1-001 - ENSP00000484472 [100%]
EPPK1-201 - ENSP00000456124 [100%]
  EPPK1-001 - ENSP00000484472 [100%]
EPPK1-201 - ENSP00000456124 [100%]
  EPPK1-001 - ENSP00000484472 [100%]
EPPK1-201 - ENSP00000456124 [100%]
 

Other gene match

      
ANTIGEN VIEW
EPPK1-001
EPPK1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.