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Antibody staining overlaps with antibody HPA066764.
Validated
Antibody staining overlaps with antibody HPA047869.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line PC-3 shows localization to nucleus & nucleoli.
Immunofluorescent staining of human cell line PC-3 shows localization to nucleus & nucleoli.
Antibody dilution
1:145
1:9
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA047869
Antibody HPA066764
Standard validation
Approved
Figure description
Immunohistochemical staining of human testis shows nuclear positivity in seminiferus ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 20 cell types
Retrieval
HIER pH6
Antibody dilution
1:700
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA047869
Antibody HPA066764
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
83.6, 24.9
83.6
Antibody dilution
1:730
1:80
PROTEIN ARRAY
Antibody HPA047869
Antibody HPA066764
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.