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PECAM1

ANTIBODY INFORMATION
 
 

Antibody HPA004690

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA004690  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 3  

References

 1  

Proper citation

 Atlas Antibodies Cat#HPA004690, RRID:AB_1078462  

VALIDATION SUMMARY
 
 

Antibody HPA004690

  
Immunohistochemistry


Validated
  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004690

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human duodenum shows strong membranous positivity in epithelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 10 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA004690

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 82.5  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA004690

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA004690

  

Antigen

 Recombinant protein fragment  

Length (aa)

 141  

Antigen sequence

 
APPANFTIQKEDTIVSQTQDFTKIASKSDSGTYICTAGIDKVVKKSNTVQ
IVVCEMLSQPRISYDAQFEVIKGQTIEVRCESISGTLPISYQLLKTSKVL
ENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVL
 

Matching transcripts

 PECAM1-001 - ENSP00000457421 [100%]
 

Other gene match

  
ANTIGEN VIEW
PECAM1-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA004690

  

1.

 Scalable in situ hybridization on tissue arrays for validation of novel cancer and tissue-specific biomarkers
Kiflemariam S et al
PLoS One 2012;7(3):e32927
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.