Fields »
ABOUT
HELP
BLOG
RBP3

ANTIBODY INFORMATION
 
 

Antibody HPA041301

  

Antibody HPA044239

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041301   HPA044239  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA041301, RRID:AB_2677398   Atlas Antibodies Cat#HPA044239, RRID:AB_2678868  

VALIDATION SUMMARY
 
 

Antibody HPA041301

  

Antibody HPA044239

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041301

  

Antibody HPA044239

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor cells.   Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor cells.  

Expression

 RNA: detected in 0 tissues
Protein: detected in 2 cell types		   RNA: detected in 0 tissues
Protein: detected in 12 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1000   1:500  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.  

WESTERN BLOT
 
 

Antibody HPA041301

  

Antibody HPA044239

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 135.4   135.4  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA041301

  

Antibody HPA044239

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA041301

  

Antibody HPA044239

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 100   98  

Antigen sequence

 
GVEPDITVPMSEALSIAQDIVALRAKVPTVLQTAGKLVADNYASAELGAK
MATKLSGLQSRYSRVTSEVALAEILGADLQMLSGDPHLKAAHIPENAKDR
  
EDNIGYLRFDMFGDGELLTQVSRLLVEHIWKKIMHTDAMIIDMRFNIGGP
TSSIPILCSYFFDEGPPVLLDKIYSRPDDSVSELWTHAQVVGERYGSK
 

Matching transcripts

 RBP3-001 - ENSP00000463151 [100%]
  RBP3-001 - ENSP00000463151 [100%]
 

Other gene match

    
ANTIGEN VIEW
RBP3-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.