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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
Antibody dilution
1:102
1:29
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA035957
Antibody HPA035958
Antibody HPA044867
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate nuclear membranous positivity in glandular cells.
Expression
RNA: detected in 7 tissues Protein: detected in 61 cell types
RNA: detected in 7 tissues Protein: detected in 70 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:675
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035957
Antibody HPA035958
Antibody HPA044867
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403503)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403503)
Target mass (kDa)
37
37, 25.7
37, 25.7
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA035957
Antibody HPA035958
Antibody HPA044867
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
