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ANTIBODY INFORMATION
 
 

Antibody HPA043355

  

Antibody HPA048460

  

Antibody CAB025749

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA043355   HPA048460   AF2927  

Host species

 Rabbit   Rabbit   Goat  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 12   12   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA043355, RRID:AB_2678442   Atlas Antibodies Cat#HPA048460, RRID:AB_2680403    

VALIDATION SUMMARY
 
 

Antibody HPA043355

  

Antibody HPA048460

  

Antibody CAB025749

  
Immunohistochemistry


Validated

Validated
  

Validated

Validated
   Supported  
Western blot

Uncertain   Supported   Uncertain  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043355

  

Antibody HPA048460

  

Antibody CAB025749

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

 
   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA048460 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA043355 across 74 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in spermatids.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.  

Expression

 RNA: detected in 2 tissues
Protein: detected in 1 cell types		   RNA: detected in 2 tissues
Protein: detected in 1 cell types		   RNA: detected in 2 tissues
Protein: detected in 9 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:7500   1:1800   1:450  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA043355

  

Antibody HPA048460

  

Antibody CAB025749

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 45   45   45, 16.6  

Antibody dilution

 1:250   1:550   1:500  

PROTEIN ARRAY
 
 

Antibody HPA043355

  

Antibody HPA048460

  

Antibody CAB025749

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:12000   1:4400    

ANTIGEN INFORMATION
 
 

Antibody HPA043355

  

Antibody HPA048460

  

Antibody CAB025749

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 67   100    

Antigen sequence

 
FLITDDEEYADNFGKATWGETKGPEREMDAIQAKEEMKKAREEEEEELLS
GKINRHEHYFNQFHRRN
  
SIEYDWNLTSLKKETSPAESKDWEQTKDNKAQDWEKHFLDASTSKQSDWN
GDLDGDWPAPMLQKPPYQDGLKPEGIHKGVWLHRKMKNTDYLTQYDLSEF
   

Matching transcripts

 CALR3-001 - ENSP00000269881 [100%]
  CALR3-001 - ENSP00000269881 [99%]
   

Other gene match

      
ANTIGEN VIEW
CALR3-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.