We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:66
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA046969
Antibody HPA047745
Antibody HPA052553
Standard validation
Uncertain
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules and strong nuclear staining in cells in glomeruli.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells seminiferus ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subsets of cells cells in seminiferous ducts.
Expression
RNA: detected in 36 tissues Protein: detected in 34 cell types
RNA: detected in 36 tissues Protein: detected in 36 cell types
RNA: detected in 36 tissues Protein: detected in 33 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:100
1:25
1:150
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA046969
Antibody HPA047745
Antibody HPA052553
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
158.7, 62.3, 62.2, 62.1, 56.4
62.3, 62.2, 62.1
62.3, 62.2, 62.1
Antibody dilution
1:330
1:110
1:500
PROTEIN ARRAY
Antibody HPA046969
Antibody HPA047745
Antibody HPA052553
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
