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Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:66
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA046969
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules and strong nuclear staining in cells in glomeruli.
Expression
RNA: detected in 15 tissues Protein: detected in 34 cell types
Retrieval
HIER pH6
Antibody dilution
1:100
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA046969
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
158.7, 62.3, 62.2, 62.1, 56.4
Antibody dilution
1:330
PROTEIN ARRAY
Antibody HPA046969
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
