ACACA - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

ACACA

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA036650

Antibody HPA063018

Antibody CAB013715

Provider

Atlas Antibodies
Sigma-Aldrich

Origene

Product name

HPA036650

HPA063018

2098.00.02

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA036650, RRID:AB_2675228

Atlas Antibodies Cat#HPA063018, RRID:AB_2684922

VALIDATION SUMMARY

Antibody HPA036650

Antibody HPA063018

Antibody CAB013715

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA036650

Antibody HPA063018

Antibody CAB013715

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center, cytosol & actin filaments.

Antibody dilution

1:72

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA036650

Antibody HPA063018

Antibody CAB013715

Standard validation

Approved

Figure description

Immunohistochemical staining of human breast shows cytoplasmic positivity in adipocytes.

Immunohistochemical staining of human testis shows strong positivity in Leydig cells.

Expression

RNA: detected in 36 tissues
Protein: detected in 67 cell types

RNA: detected in 36 tissues
Protein: detected in 70 cell types

Retrieval

HIER pH6

Antibody dilution

1:20

1:350

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA036650

Antibody HPA063018

Antibody CAB013715

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

270, 265.6, 13.1, 12.1

270, 265.6, 259.7, 257.2, 114.6, 13.1, 12.1, 10.8, 10.6, 4.7

Antibody dilution

1:250

1:80

1:500

PROTEIN ARRAY

Antibody HPA036650

Antibody HPA063018

Antibody CAB013715

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA036650

Antibody HPA063018

Antibody CAB013715

Antigen

Recombinant protein fragment

Genetic immunization

Length (aa)

Antigen sequence

MDEPSPLAQPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLS
PASVGSDTLSDL

MDEPSPLAQPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLS
PASVGSDTLSDL

Matching transcripts

ACACA-001 - ENSP00000483300 [100%]
ACACA-004 - ENSP00000477912 [100%]
ACACA-009 - ENSP00000478547 [100%]
ACACA-019 - ENSP00000483852 [100%]

Other gene match

ANTIGEN VIEW

ACACA-001	ACACA-004	ACACA-009	ACACA-019

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.