Fields »
ABOUT
HELP
BLOG
RP11-295P9.13

ANTIBODY INFORMATION
 
 

Antibody HPA047950

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA047950  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 PRPF18 - ENSG00000165630 [100%]
  

Released in version

 13  

References

  

Proper citation

 Atlas Antibodies Cat#HPA047950, RRID:AB_2680221  

VALIDATION SUMMARY
 
 

Antibody HPA047950

  
Immunohistochemistry

Uncertain  
Western blot

Supported  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047950

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human stomach shows moderate nuclear positivity in glandular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 62 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:900  

Literature conformity

  

RNA consistency

 No internal RNA expression data available for correlation.  

WESTERN BLOT
 
 

Antibody HPA047950

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 39.9, 28.9, 23.1  

Antibody dilution

 1:380  

PROTEIN ARRAY
 
 

Antibody HPA047950

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:150000  

ANTIGEN INFORMATION
 
 

Antibody HPA047950

  

Antigen

 Recombinant protein fragment  

Length (aa)

 82  

Antigen sequence

 
EPGEEDTQNDLKVHEENTTIEELEALGESLGKGDDHKDMDIITKFLKFLL
GVWAKELNAREDYVKRSVQGKLNSATQKQTES
 

Matching transcripts

 RP11-295P9.13-003 - ENSP00000473200 [100%]
 

Other gene match

 PRPF18 - ENSG00000165630 [100%]
  
ANTIGEN VIEW
RP11-295P9.13-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.