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GO:0000350 [generation of catalytic spliceosome for second transesterification step] GO:0000386 [second spliceosomal transesterification activity] GO:0005681 [spliceosomal complex] GO:0005682 [U5 snRNP] GO:0008380 [RNA splicing] GO:0046540 [U4/U6 x U5 tri-snRNP complex] GO:0071021 [U2-type post-spliceosomal complex] GO:0071048 [nuclear retention of unspliced pre-mRNA at the site of transcription]