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Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA031661 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA031660 across 74 cell types.
Standard validation
Uncertain
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules.
Immunohistochemical staining of human colon shows strong cytoplasmic(granular pattern) positivity in glandular cells.
Immunohistochemical staining of human kidney shows distinct cytoplasmic positivity with a granular pattern in tubular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 28 cell types
RNA: detected in 37 tissues Protein: detected in 44 cell types
RNA: detected in 37 tissues Protein: detected in 52 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:8
1:15
1:10
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031659
Antibody HPA031660
Antibody HPA031661
Standard validation
Uncertain
No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410345)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
54.1
54.1
54.1
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA031659
Antibody HPA031660
Antibody HPA031661
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.