DYRK4 - Antibodies - The Human Protein Atlas

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DYRK4

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA028065

Antibody HPA056073

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA028065

HPA056073

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA028065, RRID:AB_10601212

Atlas Antibodies Cat#HPA056073, RRID:AB_2683028

VALIDATION SUMMARY

Antibody HPA028065

Antibody HPA056073

Immunocytochemistry

Immunohistochemistry

Uncertain

Western blot

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA028065

Antibody HPA056073

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA056073.

Validated

Antibody staining overlaps with antibody HPA028065.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.

Immunofluorescent staining of human cell line MCF7 shows localization to vesicles.

Antibody dilution

1:106

1:56

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA028065

Antibody HPA056073

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Immunohistochemical staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.

Expression

RNA: detected in 35 tissues
Protein: detected in 72 cell types

RNA: detected in 35 tissues
Protein: detected in 65 cell types

Retrieval

HIER pH6

Antibody dilution

1:125

1:40

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA028065

Antibody HPA056073

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

72.5, 59.6

Antibody dilution

1:250

1:190

PROTEIN ARRAY

Antibody HPA028065

Antibody HPA056073

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:2250

ANTIGEN INFORMATION

Antibody HPA028065

Antibody HPA056073

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

AELYTGYPLFPGENEVEQLACIMEVLGLPPAGFIQTASRRQTFFDSKGFP
KNITNNRGKKRYPDSKDLTMVLKTYDTSFLDFLRRCLVWEP

GDQQDCLQHGADTVQLPQLVDAPKKSEAAVGAEVSMTSPGQSKNFSLKNT
NV

Matching transcripts

DYRK4-001 - ENSP00000441755 [100%]
DYRK4-003 - ENSP00000439697 [100%]
DYRK4-201 - ENSP00000010132 [100%]

Other gene match

ANTIGEN VIEW

DYRK4-001	DYRK4-003	DYRK4-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.