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ANTIBODY INFORMATION
 
 

Antibody HPA003315

  

Antibody HPA064736

  

Antibody CAB017118

  

Antibody CAB068930

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Origene
   Abnova
  

Product name

 HPA003315   HPA064736   2354   H00001634-M01  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   msAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity   Not known  

Other gene match

        

Released in version

 3   16   4   16  

References

 4        

Proper citation

 Atlas Antibodies Cat#HPA003315, RRID:AB_1078639   Atlas Antibodies Cat#HPA064736, RRID:AB_2685340     Abnova Corporation Cat#H00001634-M01, RRID:AB_425394  

VALIDATION SUMMARY
 
 

Antibody HPA003315

  

Antibody HPA064736

  

Antibody CAB017118

  

Antibody CAB068930

  
Immunohistochemistry

Supported   Supported   Supported   Supported  
Western blot

Supported   Supported   Uncertain   Uncertain  
Protein array

Supported   Approved      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003315

  

Antibody HPA064736

  

Antibody CAB017118

  

Antibody CAB068930

  

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human ovary shows extracellular positivity.   Immunohistochemical staining of human ovary shows extracellular positivity.   Immunohistochemical staining of human ovary shows extracellular positivity.   Immunohistochemical staining of human ovary shows extracellular positivityY.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 16 cell types		   RNA: detected in 37 tissues
Protein: detected in 51 cell types		   RNA: detected in 37 tissues
Protein: detected in 20 cell types		   RNA: detected in 37 tissues
Protein: detected in 34 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:200   1:700   1:1000   1:16000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003315

  

Antibody HPA064736

  

Antibody CAB017118

  

Antibody CAB068930

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403339)		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403339)		      

Target mass (kDa)

 39.7, 27.4, 23.3, 19.3   39.7   39.7, 27.4, 23.3, 20.2, 20.1, 19.6, 19.3, 19.2, 13.3, 11.2, 10.2, 9.8, 8.3, 5.8   39.7, 27.4, 23.3, 20.2, 20.1, 19.6, 19.3, 19.2, 13.3, 11.2, 10.2, 9.8, 8.3, 5.8  

Antibody dilution

 1:250   1:125   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA003315

  

Antibody HPA064736

  

Antibody CAB017118

  

Antibody CAB068930

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:2000   1:1650      

ANTIGEN INFORMATION
 
 

Antibody HPA003315

  

Antibody HPA064736

  

Antibody CAB017118

  

Antibody CAB068930

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Genetic immunization   Recombinant protein  

Length (aa)

 127   85      

Antigen sequence

 
TNITSIPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISA
VDNGSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGS
SDFCPPGHNTKKASYSGVSLFSNPVQY
  
VSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHENEITKVRKVT
FNGLNQMIVIELGTNPLKSSGIENGAFQGMKKLSY
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					DCN-001 - ENSP00000052754 [100%] 
DCN-002 - ENSP00000447654 [100%] 
DCN-018 - ENSP00000413723 [100%] 
DCN-201 - ENSP00000376862 [100%] 
DCN-006 - ENSP00000447674 [94%] 
DCN-202 - ENSP00000401021 [94%] 
DCN-007 - ENSP00000446530 [84%] 

											 
																						DCN-001 - ENSP00000052754 [100%] 
DCN-002 - ENSP00000447654 [100%] 
DCN-201 - ENSP00000376862 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
DCN-001
DCN-002
DCN-006
DCN-007
DCN-018
DCN-201
DCN-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA003315

												
										 
																				
											
											
											
Antibody HPA064736

												
										 
																				
											
											
											
Antibody CAB017118

												
										 
																				
											
											
											
Antibody CAB068930

												
										 
																		

																	

										
											
1.

												
																						Proteomic analyses reveal high expression of decorin and endoplasmin (HSP90B1) are associated with breast cancer metastasis and decreased survival

	Cawthorn TR et al

	PLoS One 2012;7(2):e30992

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Stromal expression of decorin, Semaphorin6D, SPARC, Sprouty1 and Tsukushi in developing prostate and decreased levels of decorin in prostate cancer

	Henke A et al

	PLoS One 2012;7(8):e42516

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																						CD99 is a novel prognostic stromal marker in non-small cell lung cancer

	Edlund K et al

	Int J Cancer 2012;131(10):2264-73

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
4.

												
																						Decreased decorin expression in the tumor microenvironment

	Bozoky B et al

	Cancer Med 2014;3(3):485-91

			
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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