MVP - Antibodies - The Human Protein Atlas

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MVP

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA002321

Antibody HPA064740

Antibody CAB002752

Antibody CAB022717

Provider

Atlas Antibodies
Sigma-Aldrich

Chemicon

Product name

HPA002321

HPA064740

MAB4126

MAB4141

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Serum

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA002321, RRID:AB_1079277

Atlas Antibodies Cat#HPA064740, RRID:AB_2685342

VALIDATION SUMMARY

Antibody HPA002321

Antibody HPA064740

Antibody CAB002752

Antibody CAB022717

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA002321

Antibody HPA064740

Antibody CAB002752

Antibody CAB022717

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Antibody dilution

1:42

1:4

1:100

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA002321

Antibody HPA064740

Antibody CAB002752

Antibody CAB022717

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA064740 across 45 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA002321 across 45 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA064740 across 67 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA002321 across 67 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human small intestine shows cytoplasmic and membrane positivity in glandular cells.

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 51 cell types

RNA: detected in 37 tissues
Protein: detected in 55 cell types

RNA: detected in 37 tissues
Protein: detected in 60 cell types

RNA: detected in 37 tissues
Protein: detected in 56 cell types

Retrieval

HIER pH6

Antibody dilution

1:250

1:2000

1:10

1:400

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA002321

Antibody HPA064740

Antibody CAB002752

Antibody CAB022717

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

99.3

99.3, 25.1, 16.9, 16.5, 12.4, 10.9, 5, 4.5

Antibody dilution

1:250

1:300

1:500

PROTEIN ARRAY

Antibody HPA002321

Antibody HPA064740

Antibody CAB002752

Antibody CAB022717

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2000

1:3650

ANTIGEN INFORMATION

Antibody HPA002321

Antibody HPA064740

Antibody CAB002752

Antibody CAB022717

Antigen

Recombinant protein fragment

Synthetic peptide

Recombinant protein

Length (aa)

120

Antigen sequence

VFEEVLDLVDAVILTEKTALHLRARRNFRDFRGVSRRTGEEWLVTVQDTE
AHVPDVHEEVLGVVPITTLGPHNYCVILDPVGPDGKNQLGQKRVVKGEKS
FFLQPGEQLEQGIQDVYVLS

KVSHQAGDHWLIRGPLEYVPSAKVEVVEERQAIPLDENEGIYVQDVKTGK
VRAVIGSTYMLTQDEVLWEKELPPGVEELLNKGQDPLAD

Matching transcripts

MVP-001 - ENSP00000349977 [100%]
MVP-201 - ENSP00000378760 [100%]

Other gene match

ANTIGEN VIEW

MVP-001	MVP-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.