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Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA022039 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA005781 across 46 cell lines.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human skin shows strong nuclear and cytoplasmic positivity in epidermal cells.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 35 tissues Protein: detected in 47 cell types
RNA: detected in 35 tissues Protein: detected in 43 cell types
RNA: detected in 35 tissues Protein: detected in 37 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:50
1:175
1:100
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA005781
Antibody HPA006050
Antibody HPA022039
Antibody CAB010111
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416710)
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
90.4, 24.9, 23.8, 21, 15.9
90.4, 24.9, 23.8, 21, 15.9
90.4
90.4, 24.9, 23.8, 23.1, 21, 15.9
Antibody dilution
1:250
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA005781
Antibody HPA006050
Antibody HPA022039
Antibody CAB010111
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:500
1:1000
1:500
ANTIGEN INFORMATION
Antibody HPA005781
Antibody HPA006050
Antibody HPA022039
Antibody CAB010111
Antigen
Recombinant protein fragment
Recombinant protein fragment
Recombinant protein fragment
Recombinant protein
Length (aa)
102
102
110
Antigen sequence
MSLQVLNDKNVSNEKNTENCDFLFSPPEVTGRSSVLRVSQKENVPPKNLA
KAMKVTFQTPLRDPQTHRILSPSMASKLEAPFTQDDTLGLENSHPVWTQK
EN
MSLQVLNDKNVSNEKNTENCDFLFSPPEVTGRSSVLRVSQKENVPPKNLA
KAMKVTFQTPLRDPQTHRILSPSMASKLEAPFTQDDTLGLENSHPVWTQK
EN
