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RUFY3

ANTIBODY INFORMATION
 
 

Antibody HPA022952

  

Antibody HPA022970

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA022952   HPA022970  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   5  

References

    

Proper citation

 Atlas Antibodies Cat#HPA022952, RRID:AB_2671005   Atlas Antibodies Cat#HPA022970, RRID:AB_1856307  

VALIDATION SUMMARY
 
 

Antibody HPA022952

  

Antibody HPA022970

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA022952

  

Antibody HPA022970

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to cytosol.  

Antibody dilution

   1:100  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA022952

  

Antibody HPA022970

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 39 cell types		   RNA: detected in 37 tissues
Protein: detected in 49 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:800  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA022952

  

Antibody HPA022970

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 70.6, 53   70.6, 64.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA022952

  

Antibody HPA022970

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA022952

  

Antibody HPA022970

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 83   113  

Antigen sequence

 
MSALTPPTDMPTPTTDKITQAAMETIYLCKFRVSMDGEWLCLRELDDISL
TPDPEPTHEDPNYLMANERMNLMNMAKLSIKGL
  
MLEKDVCEKQDALVSLRQQLDDLRALKHELAFKLQSSDLGVKQKSELNSR
LEEKTNQMAATIKQLEQRLRQAERSRQSAELDNRLFKQDFGDKINSLQLE
VEELTRQRNQLEL
 

Matching transcripts

 RUFY3-001 - ENSP00000226328 [100%]
RUFY3-002 - ENSP00000370394 [100%]
  RUFY3-002 - ENSP00000370394 [100%]
RUFY3-003 - ENSP00000425400 [100%]
 

Other gene match

    
ANTIGEN VIEW
RUFY3-001
RUFY3-002
RUFY3-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.