We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Fields »
 

Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
ABOUT
HELP
BLOG
  • THE HUMAN PROTEOME
  • THE HUMAN TISSUES
  • THE HUMAN CELL
  • HUMAN PATHOLOGY
  • PROTEIN CLASSES
  • PROTEIN EVIDENCE
    • NEWS
    • BLOG
    • EVENTS
    • PRESS ROOM
    • LEARN
    • DICTIONARY
    • METHODS
    • CELL LINES
  • THE PROJECT
  • INTRODUCTION
  • ORGANIZATION
  • PUBLICATIONS
  • PUBLICATION DATA
  • ANTIBODY SUBMISSION
  • ANTIBODY AVAILABILITY
  • LINKS
  • CONTACT
  • TECHNICAL DATA
  • ANTIBODY VALIDATION
  • ASSAYS & ANNOTATION
  • DISCLAIMER
  • DOWNLOADABLE DATA
  • HELP & FAQ
  • LICENCE & CITATION
  • PRIVACY STATEMENT
  • RELEASE HISTORY
CD74
  • premium

    TISSUE

  • premium

    CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

CD74

Gene description

CD74 molecule, major histocompatibility complex, class II invariant chain

Protein class

Cancer-related genes
CD markers
Disease related genes
Predicted membrane proteins

Predicted localization

Membrane

Number of transcripts

5
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Cell line enhanced (CAPAN-2, Daudi, HDLM-2, REH)
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic and membranous expression in macrophages and immune cells in several tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data. Antibody staining in cells/structures not annotated, view images.

Reliability score

Supported based on 2 antibodies.
HPA010592 , CAB002506
SHOW MORE

PROGNOSTIC SUMMARY

Prognostic marker in endometrial cancer (favourable) and breast cancer (favourable).
Endometrial cancer p<0.001
Breast cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA010592
CAB002506

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most malignant tissues showed moderate to strong cytoplasmic positivity. Malignant gliomas, skin and testicular cancers were generally negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancer types displayed cytoplasmic staining of moderate intensity, while the several cancer tissues were negative. Variations of intensities were observed within the same tumor type.

GENE INFORMATION

Gene name

CD74 (HGNC Symbol)

Synonyms

DHLAG

Description

CD74 molecule, major histocompatibility complex, class II invariant chain (HGNC Symbol)

Entrez gene summary

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Chromosome

5

Cytoband

q33.1

Chromosome location (bp)

150401637 - 150412929

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000019582 (version 83.38)

Entrez gene

972

UniProt

P04233 (UniProt - Evidence at protein level)

neXtProt

NX_P04233

Antibodypedia

CD74 antibodies

PROTEIN BROWSER

CD74-001
CD74-002
CD74-003
CD74-004
CD74-011

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

CD74-001
ENSP00000230685
ENST00000353334
P04233 [Direct mapping]
HLA class II histocompatibility antigen gamma chain
Show all
CD markers
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Cancer-related genes
   Candidate cancer biomarkers
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Translocations
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000139 [Golgi membrane]
GO:0001516 [prostaglandin biosynthetic process]
GO:0001540 [beta-amyloid binding]
GO:0001961 [positive regulation of cytokine-mediated signaling pathway]
GO:0002792 [negative regulation of peptide secretion]
GO:0004896 [cytokine receptor activity]
GO:0005515 [protein binding]
GO:0005622 [intracellular]
GO:0005765 [lysosomal membrane]
GO:0005773 [vacuole]
GO:0005886 [plasma membrane]
GO:0006461 [protein complex assembly]
GO:0006886 [intracellular protein transport]
GO:0006955 [immune response]
GO:0007165 [signal transduction]
GO:0008283 [cell proliferation]
GO:0009986 [cell surface]
GO:0012507 [ER to Golgi transport vesicle membrane]
GO:0016020 [membrane]
GO:0016021 [integral component of membrane]
GO:0016064 [immunoglobulin mediated immune response]
GO:0019882 [antigen processing and presentation]
GO:0019883 [antigen processing and presentation of endogenous antigen]
GO:0019886 [antigen processing and presentation of exogenous peptide antigen via MHC class II]
GO:0019955 [cytokine binding]
GO:0023026 [MHC class II protein complex binding]
GO:0030658 [transport vesicle membrane]
GO:0030666 [endocytic vesicle membrane]
GO:0030669 [clathrin-coated endocytic vesicle membrane]
GO:0030890 [positive regulation of B cell proliferation]
GO:0032588 [trans-Golgi network membrane]
GO:0035692 [macrophage migration inhibitory factor receptor complex]
GO:0035718 [macrophage migration inhibitory factor binding]
GO:0042289 [MHC class II protein binding]
GO:0042613 [MHC class II protein complex]
GO:0042658 [MHC class II protein binding, via antigen binding groove]
GO:0042802 [identical protein binding]
GO:0043030 [regulation of macrophage activation]
GO:0043066 [negative regulation of apoptotic process]
GO:0043202 [lysosomal lumen]
GO:0043518 [negative regulation of DNA damage response, signal transduction by p53 class mediator]
GO:0044183 [protein binding involved in protein folding]
GO:0045058 [T cell selection]
GO:0048146 [positive regulation of fibroblast proliferation]
GO:0050731 [positive regulation of peptidyl-tyrosine phosphorylation]
GO:0060907 [positive regulation of macrophage cytokine production]
GO:0070062 [extracellular exosome]
GO:0070374 [positive regulation of ERK1 and ERK2 cascade]
GO:0071556 [integral component of lumenal side of endoplasmic reticulum membrane]
GO:0090023 [positive regulation of neutrophil chemotaxis]
GO:1902166 [negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator]
GO:2000343 [positive regulation of chemokine (C-X-C motif) ligand 2 production]
Show all
232 aa
26.4 kDa
No 1
CD74-002
ENSP00000367026
ENST00000377795
P04233 [Direct mapping]
HLA class II histocompatibility antigen gamma chain
Show all
CD markers
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Cancer-related genes
   Candidate cancer biomarkers
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Translocations
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000139 [Golgi membrane]
GO:0001516 [prostaglandin biosynthetic process]
GO:0001540 [beta-amyloid binding]
GO:0001961 [positive regulation of cytokine-mediated signaling pathway]
GO:0002792 [negative regulation of peptide secretion]
GO:0004896 [cytokine receptor activity]
GO:0005515 [protein binding]
GO:0005622 [intracellular]
GO:0005765 [lysosomal membrane]
GO:0005773 [vacuole]
GO:0005886 [plasma membrane]
GO:0006461 [protein complex assembly]
GO:0006886 [intracellular protein transport]
GO:0006955 [immune response]
GO:0007165 [signal transduction]
GO:0008283 [cell proliferation]
GO:0009986 [cell surface]
GO:0012507 [ER to Golgi transport vesicle membrane]
GO:0016020 [membrane]
GO:0016021 [integral component of membrane]
GO:0016064 [immunoglobulin mediated immune response]
GO:0019882 [antigen processing and presentation]
GO:0019883 [antigen processing and presentation of endogenous antigen]
GO:0019886 [antigen processing and presentation of exogenous peptide antigen via MHC class II]
GO:0019955 [cytokine binding]
GO:0023026 [MHC class II protein complex binding]
GO:0030658 [transport vesicle membrane]
GO:0030666 [endocytic vesicle membrane]
GO:0030669 [clathrin-coated endocytic vesicle membrane]
GO:0030890 [positive regulation of B cell proliferation]
GO:0032588 [trans-Golgi network membrane]
GO:0035692 [macrophage migration inhibitory factor receptor complex]
GO:0035718 [macrophage migration inhibitory factor binding]
GO:0042289 [MHC class II protein binding]
GO:0042613 [MHC class II protein complex]
GO:0042658 [MHC class II protein binding, via antigen binding groove]
GO:0042802 [identical protein binding]
GO:0043030 [regulation of macrophage activation]
GO:0043066 [negative regulation of apoptotic process]
GO:0043202 [lysosomal lumen]
GO:0043518 [negative regulation of DNA damage response, signal transduction by p53 class mediator]
GO:0044183 [protein binding involved in protein folding]
GO:0045058 [T cell selection]
GO:0048146 [positive regulation of fibroblast proliferation]
GO:0050731 [positive regulation of peptidyl-tyrosine phosphorylation]
GO:0060907 [positive regulation of macrophage cytokine production]
GO:0070062 [extracellular exosome]
GO:0070374 [positive regulation of ERK1 and ERK2 cascade]
GO:0071556 [integral component of lumenal side of endoplasmic reticulum membrane]
GO:0090023 [positive regulation of neutrophil chemotaxis]
GO:1902166 [negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator]
GO:2000343 [positive regulation of chemokine (C-X-C motif) ligand 2 production]
Show all
160 aa
18.3 kDa
No 1
CD74-003
ENSP00000009530
ENST00000009530
P04233 [Direct mapping]
HLA class II histocompatibility antigen gamma chain
Show all
CD markers
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Cancer-related genes
   Candidate cancer biomarkers
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Translocations
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000139 [Golgi membrane]
GO:0000187 [activation of MAPK activity]
GO:0001516 [prostaglandin biosynthetic process]
GO:0001540 [beta-amyloid binding]
GO:0001961 [positive regulation of cytokine-mediated signaling pathway]
GO:0002606 [positive regulation of dendritic cell antigen processing and presentation]
GO:0002792 [negative regulation of peptide secretion]
GO:0002830 [positive regulation of type 2 immune response]
GO:0002906 [negative regulation of mature B cell apoptotic process]
GO:0004896 [cytokine receptor activity]
GO:0005515 [protein binding]
GO:0005622 [intracellular]
GO:0005764 [lysosome]
GO:0005765 [lysosomal membrane]
GO:0005770 [late endosome]
GO:0005771 [multivesicular body]
GO:0005773 [vacuole]
GO:0005783 [endoplasmic reticulum]
GO:0005794 [Golgi apparatus]
GO:0005886 [plasma membrane]
GO:0006461 [protein complex assembly]
GO:0006886 [intracellular protein transport]
GO:0006952 [defense response]
GO:0006955 [immune response]
GO:0007165 [signal transduction]
GO:0008283 [cell proliferation]
GO:0009897 [external side of plasma membrane]
GO:0009986 [cell surface]
GO:0012507 [ER to Golgi transport vesicle membrane]
GO:0016020 [membrane]
GO:0016021 [integral component of membrane]
GO:0016064 [immunoglobulin mediated immune response]
GO:0019882 [antigen processing and presentation]
GO:0019883 [antigen processing and presentation of endogenous antigen]
GO:0019886 [antigen processing and presentation of exogenous peptide antigen via MHC class II]
GO:0019955 [cytokine binding]
GO:0023026 [MHC class II protein complex binding]
GO:0030658 [transport vesicle membrane]
GO:0030666 [endocytic vesicle membrane]
GO:0030669 [clathrin-coated endocytic vesicle membrane]
GO:0030890 [positive regulation of B cell proliferation]
GO:0032588 [trans-Golgi network membrane]
GO:0035691 [macrophage migration inhibitory factor signaling pathway]
GO:0035692 [macrophage migration inhibitory factor receptor complex]
GO:0035693 [NOS2-CD74 complex]
GO:0035718 [macrophage migration inhibitory factor binding]
GO:0042289 [MHC class II protein binding]
GO:0042613 [MHC class II protein complex]
GO:0042658 [MHC class II protein binding, via antigen binding groove]
GO:0042802 [identical protein binding]
GO:0043030 [regulation of macrophage activation]
GO:0043066 [negative regulation of apoptotic process]
GO:0043202 [lysosomal lumen]
GO:0043518 [negative regulation of DNA damage response, signal transduction by p53 class mediator]
GO:0044183 [protein binding involved in protein folding]
GO:0045058 [T cell selection]
GO:0045059 [positive thymic T cell selection]
GO:0045060 [negative thymic T cell selection]
GO:0045581 [negative regulation of T cell differentiation]
GO:0045582 [positive regulation of T cell differentiation]
GO:0048146 [positive regulation of fibroblast proliferation]
GO:0050731 [positive regulation of peptidyl-tyrosine phosphorylation]
GO:0050998 [nitric-oxide synthase binding]
GO:0051085 [chaperone mediated protein folding requiring cofactor]
GO:0060907 [positive regulation of macrophage cytokine production]
GO:0070062 [extracellular exosome]
GO:0070374 [positive regulation of ERK1 and ERK2 cascade]
GO:0071556 [integral component of lumenal side of endoplasmic reticulum membrane]
GO:0090023 [positive regulation of neutrophil chemotaxis]
GO:1902166 [negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator]
GO:2000343 [positive regulation of chemokine (C-X-C motif) ligand 2 production]
Show all
296 aa
33.5 kDa
No 1
CD74-004
ENSP00000430614
ENST00000518797
H0YBZ2 [Direct mapping]
HLA class II histocompatibility antigen gamma chain
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005622 [intracellular]
GO:0006886 [intracellular protein transport]
GO:0006955 [immune response]
GO:0016020 [membrane]
GO:0016021 [integral component of membrane]
GO:0019882 [antigen processing and presentation]
GO:0042289 [MHC class II protein binding]
Show all
316 aa
35 kDa
No 1
CD74-011
ENSP00000429024
ENST00000524315
E7ESL3 [Direct mapping]
HLA class II histocompatibility antigen gamma chain
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005622 [intracellular]
GO:0006886 [intracellular protein transport]
GO:0006955 [immune response]
GO:0016020 [membrane]
GO:0016021 [integral component of membrane]
GO:0019882 [antigen processing and presentation]
GO:0042289 [MHC class II protein binding]
Show all
152 aa
17.2 kDa
No 1

Contact

  • BLOG
  • PRESS ROOM
  • contact@proteinatlas.org

The Project

  • INTRODUCTION
  • ORGANIZATION
  • PUBLICATIONS
  • DOWNLOADABLE DATA

The Human Protein Atlas

  • ANTIBODY AVAILABILITY
  • LICENCE & CITATION
  • HELP & FAQ
The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.