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RABEP1

ANTIBODY INFORMATION
 
 

Antibody HPA019669

  

Antibody HPA024235

  

Antibody HPA024691

  

Antibody CAB004549

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA019669   HPA024235   HPA024691   sc-46658  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

        

Released in version

 5   5   5   2  

References

        

Proper citation

 Atlas Antibodies Cat#HPA019669, RRID:AB_1856019   Atlas Antibodies Cat#HPA024235, RRID:AB_1856020   Atlas Antibodies Cat#HPA024691, RRID:AB_1856021   Santa Cruz Biotechnology Cat#sc-46658, RRID:AB_628192  

VALIDATION SUMMARY
 
 

Antibody HPA019669

  

Antibody HPA024235

  

Antibody HPA024691

  

Antibody CAB004549

  
Immunocytochemistry

  Supported      
Immunohistochemistry

Supported  

Validated
  

Validated
  

Validated
  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Supported   Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA019669

  

Antibody HPA024235

  

Antibody HPA024691

  

Antibody CAB004549

  

Standard validation

   Supported      

       

Figure description

   Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.      

Antibody dilution

   1:100      

Literature conformity

   The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019669

  

Antibody HPA024235

  

Antibody HPA024691

  

Antibody CAB004549

  

Formal validation: Orthogonal

      
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

     
 

Figure description

       Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Formal validation: Independent

  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
 
   

Figure description

   Distribution of protein expression (antibody staining).
Pearson correlation with HPA024691 across 45 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA024235 across 45 cell lines.		    
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human lymph node shows strong positivity.   Immunohistochemical staining of human tonsil shows strong positivity.   Immunohistochemical staining of human lymph node shows strong positivity in a subset of germinal center cells.   Immunohistochemical staining of human lymph node shows distinct positivity.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 70 cell types		   RNA: detected in 37 tissues
Protein: detected in 70 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		   RNA: detected in 37 tissues
Protein: detected in 56 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:175   1:300   1:400   1:300  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA019669

  

Antibody HPA024235

  

Antibody HPA024691

  

Antibody CAB004549

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Testis		  

Target mass (kDa)

 99.3, 95.5   99.3, 95.5   99.3, 95.5   99.3, 95.5, 20.6, 17.4, 12.5, 12  

Antibody dilution

 1:250   1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA019669

  

Antibody HPA024235

  

Antibody HPA024691

  

Antibody CAB004549

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA019669

  

Antibody HPA024235

  

Antibody HPA024691

  

Antibody CAB004549

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 96   120   97    

Antigen sequence

 
SREQVSEELVRLQKDNDSLQGKHSLHVSLQQAEDFILPDTTEALRELVLK
YREDIINVRTAADHVEEKLKAEILFLKEQIQAEQCLKENLEETLQL
  
ERQQHNQLKHTWQKANDQFLESQRLLMRDMQRMEIVLTSEQLRQVEELKK
KDQEDDEQQRLNKRKDHKKADVEEEIKIPVVCALTQEESSAQLSNEEEHL
DSTRGSVHSLDAGLLLPSGD
  
VHNAGNKLGRRCDMCSNYEKQLQGIQIQEAETRDQVKKLQLMLRQANDQL
EKTMKDKQELEDFIKQSSEDSSHQISALVLRAQASEILLEELQQGLS
  

											 
																				

																		

										
											
Matching transcripts

												
																					RABEP1-001 - ENSP00000445408 [100%] 
RABEP1-002 - ENSP00000339569 [100%] 

											 
																						RABEP1-001 - ENSP00000445408 [100%] 
RABEP1-002 - ENSP00000339569 [100%] 

											 
																						RABEP1-001 - ENSP00000445408 [100%] 
RABEP1-002 - ENSP00000339569 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
RABEP1-001
RABEP1-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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