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ABCF2

ANTIBODY INFORMATION
 
 

Antibody HPA020091

  

Antibody HPA021815

  

Antibody HPA030388

  

Antibody CAB020682

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA020091   HPA021815   HPA030388   3111.00.02  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

        

Released in version

 5   5   6   5  

References

        

Proper citation

 Atlas Antibodies Cat#HPA020091, RRID:AB_1844439   Atlas Antibodies Cat#HPA021815, RRID:AB_1844440   Atlas Antibodies Cat#HPA030388, RRID:AB_10601558    

VALIDATION SUMMARY
 
 

Antibody HPA020091

  

Antibody HPA021815

  

Antibody HPA030388

  

Antibody CAB020682

  
Immunocytochemistry

Approved       Approved  
Immunohistochemistry

Supported   Supported   Supported   Supported  
Western blot

Supported   Uncertain   Supported   Supported  
Protein array

Supported   Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA020091

  

Antibody HPA021815

  

Antibody HPA030388

  

Antibody CAB020682

  

Standard validation

 Approved       Approved  

       

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol.       Immunofluorescent staining of human cell line A-431 shows localization to cytosol.  

Antibody dilution

 1:200       1:44  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.       The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA020091

  

Antibody HPA021815

  

Antibody HPA030388

  

Antibody CAB020682

  

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human stomach shows moderate positivity in chief cells.   Immunohistochemical staining of human cerebellum shows distinct cytoplasmic positivity in Purkinje cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 69 cell types		   RNA: detected in 37 tissues
Protein: detected in 62 cell types		   RNA: detected in 37 tissues
Protein: detected in 42 cell types		   RNA: detected in 37 tissues
Protein: detected in 41 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:400   1:15   1:50   1:175  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA020091

  

Antibody HPA021815

  

Antibody HPA030388

  

Antibody CAB020682

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 72.4, 71.3, 32.6, 26.9   72.4, 71.3   72.4, 71.3   72.4, 71.3, 32.6, 26.9  

Antibody dilution

 1:250   1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA020091

  

Antibody HPA021815

  

Antibody HPA030388

  

Antibody CAB020682

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA020091

  

Antibody HPA021815

  

Antibody HPA030388

  

Antibody CAB020682

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Genetic immunization  

Length (aa)

 112   90   70    

Antigen sequence

 
KEAAKARQRPRKGHEENGDVVTEPQVAEKNEANGRETTEVDLLTKELEDF
EMKKAAARAVTGVLASHPNSTDVHIINLSLTFHGQELLSDTKLELNSGRR
YGLIGLNGIGKS
  
NHLDIETIDALADAINEFEGGMMLVSHDFRLIQQVAQEIWVCEKQTITKW
PGDILAYKEHLKSKLVDEEPQLTKRTHNVCTLTLASLPRP
  
CVWLEEELKTFKRILVLVSHSQDFLNGVCTNIIHMHNKKLKYYTGNYDQY
VKTRLELEENQMKRFHWEQD
  

											 
																				

																		

										
											
Matching transcripts

												
																					ABCF2-001 - ENSP00000287844 [100%] 
ABCF2-003 - ENSP00000222388 [100%] 
ABCF2-004 - ENSP00000395785 [100%] 
ABCF2-006 - ENSP00000419720 [100%] 

											 
																						ABCF2-003 - ENSP00000222388 [100%] 
ABCF2-001 - ENSP00000287844 [88%] 

											 
																						ABCF2-001 - ENSP00000287844 [100%] 
ABCF2-003 - ENSP00000222388 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ABCF2-001
ABCF2-003
ABCF2-004
ABCF2-006
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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