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ANTIBODY INFORMATION
 
 

Antibody HPA002740

  

Antibody CAB000077

  

Antibody CAB056155

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA002740   A0251   AMAb90523  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

      

Released in version

 2   1   10  

References

      

Proper citation

 Atlas Antibodies Cat#HPA002740, RRID:AB_1080277     Atlas Antibodies Cat#AMAb90523, RRID:AB_2665573  

VALIDATION SUMMARY
 
 

Antibody HPA002740

  

Antibody CAB000077

  

Antibody CAB056155

  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Uncertain   Uncertain   Supported  
Protein array

Supported      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002740

  

Antibody CAB000077

  

Antibody CAB056155

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human thyroid gland shows distinct cytoplasmic and extracellular positivity in glandular cells.   Immunohistochemical staining of human thyroid gland shows strong cytoplasmic and extra-cellular positivity in glandular cells.   Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 16 tissues
Protein: detected in 1 cell types		   RNA: detected in 16 tissues
Protein: detected in 3 cell types		   RNA: detected in 16 tissues
Protein: detected in 1 cell types		  

Retrieval

 HIER pH6   None   HIER pH6  

Antibody dilution

 1:250   1:8000   1:1000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002740

  

Antibody CAB000077

  

Antibody CAB056155

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 304.8   304.8, 134.6, 99.4, 23.7, 20.9, 19.3, 6.6   304.8, 134.6, 99.4, 23.7, 20.9, 19.3, 6.6  

Antibody dilution

 1:250   1:5000   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA002740

  

Antibody CAB000077

  

Antibody CAB056155

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA002740

  

Antibody CAB000077

  

Antibody CAB056155

  

Antigen

 Recombinant protein fragment   Native protein   Recombinant protein  

Length (aa)

 163      

Antigen sequence

 
KMCSADYADLLQTFQVFILDELTARGFCQIQVKTFGTLVSIPVCNNSSVQ
VGCLTRERLGVNVTWKSRLEDIPVASLPDLHDIERALVGKDLLGRFTDLI
QSGSFQLHLDSKTFPAETIRFLQGDHFGTSPRTWFGCSEGFYQVLTSEAS
QDGLGCVKCPEGS
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					TG-001 - ENSP00000220616 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
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