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WDR37

ANTIBODY INFORMATION
 
 

Antibody HPA037376

  

Antibody HPA037565

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA037376   HPA037565  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   7  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA037376, RRID:AB_10672789   Atlas Antibodies Cat#HPA037565, RRID:AB_10696937  

VALIDATION SUMMARY
 
 

Antibody HPA037376

  

Antibody HPA037565

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Supported  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA037376

  

Antibody HPA037565

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.   Immunofluorescent staining of human cell line U-2 OS shows localization to aggresome.  

Antibody dilution

 1:125   1:200  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037376

  

Antibody HPA037565

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 79 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:75  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA037376

  

Antibody HPA037565

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 54.7   54.7, 29.1, 27.4  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA037376

  

Antibody HPA037565

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA037376

  

Antibody HPA037565

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 109   85  

Antigen sequence

 
FRDPSIHSVNVFQGHTDTVTSAVFTVGDNVVSGSDDRTVKVWDLKNMRSP
IATIRTDSAINRINVCVGQKIIALPHDNRQVRLFDMSGVRLARLPRSSRQ
GHRRMVCCS
  
TARQTKQKRKSHSLSIRRTNSSEQERTGLPRDMLEGQDSKLPSSVRSTLL
ELFGQIEREFENLYIENLELRREIDTLNERLAAEG
 

Matching transcripts

 WDR37-001 - ENSP00000350954 [100%]
WDR37-002 - ENSP00000263150 [100%]
  WDR37-001 - ENSP00000350954 [100%]
WDR37-002 - ENSP00000263150 [100%]
WDR37-004 - ENSP00000370730 [100%]
WDR37-201 - ENSP00000478057 [100%]
 

Other gene match

    
ANTIGEN VIEW
WDR37-001
WDR37-002
WDR37-004
WDR37-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA037376

  

Antibody HPA037565

  

1.

 Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.